Zhang Qing, Zhang Xiongying, Liu Na, Liu Jia, Wang Wei, Wang Yongshun, Lei Wen, Zhao Cunzhe, Ma Wanli, Guo Shuai, Cai Huixia, Zhang Jingxiao, Liu Yufang, Shi Kemei, Zhang Wen, Ma Xiao
Qinghai Institute of Endemic Disease Prevention and Control, Xining 811602, China.
Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang 212013, China.
Vet Sci. 2025 Jun 9;12(6):565. doi: 10.3390/vetsci12060565.
Alveolar echinococcosis (AE) is a chronic and potentially fatal zoonotic parasitic disease that seriously affects the host's health. It is caused by the proliferation of larvae within the liver. Due to its long incubation period following host infection, early diagnosis of the disease is currently not feasible. Treatment options are extremely limited, with the only choice being curative surgical resection combined with benzimidazole medication. Thus, the development of early, rapid, and minimally invasive diagnostic methods is crucial for enhancing patient prognosis. This study conducted proteomic and metabolomic analyses of protein and metabolite changes in the serum of a treatment group and control group, aiming to compare the differences between them. Overall, 22 proteins showed significant differences between the treatment and control groups, primarily involved in carbohydrate metabolism, lipid metabolism, and amino acid metabolism. The upregulation of genes related to immune response and enhanced glycolysis were observed, possibly associated with the reproduction of in the liver. A total of 182 metabolites were screened to distinguish between the treatment group and control group. A significant increase in the cytochrome P450 (cP450) metabolite of arachidonic acid indicated signs of renal and splenic involvement in the treatment group. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis highlighted a strong association between amino acid metabolism and the development of AE. The observed changes in amino acid levels may provide nutrients that facilitate colonization and contribute to the pathogenesis of AE. In summary, by investigating the different characteristics of the AE and control group through proteomic ( = 4/group/time point) and metabolomic ( = 8/group/time point) analyses, potential serum biomarkers for diagnosing mice with AE were identified.
肺泡型棘球蚴病（AE）是一种慢性且可能致命的人畜共患寄生虫病，严重影响宿主健康。它由幼虫在肝脏内增殖引起。由于宿主感染后的潜伏期较长，目前该病的早期诊断尚不可行。治疗选择极为有限，唯一的选择是根治性手术切除并联合苯并咪唑类药物治疗。因此，开发早期、快速且微创的诊断方法对于改善患者预后至关重要。本研究对治疗组和对照组血清中的蛋白质和代谢物变化进行了蛋白质组学和代谢组学分析，旨在比较两者之间的差异。总体而言，治疗组和对照组之间有22种蛋白质表现出显著差异，主要涉及碳水化合物代谢、脂质代谢和氨基酸代谢。观察到与免疫反应相关基因的上调和糖酵解增强，这可能与肝脏内幼虫的繁殖有关。共筛选出182种代谢物以区分治疗组和对照组。治疗组中花生四烯酸的细胞色素P450（cP450）代谢物显著增加，表明存在肾和脾受累迹象。此外，京都基因与基因组百科全书（KEGG）通路分析突出了氨基酸代谢与AE发展之间的密切关联。观察到的氨基酸水平变化可能提供促进幼虫定植的营养物质，并有助于AE的发病机制。总之，通过蛋白质组学（每组/每个时间点 = 4）和代谢组学（每组/每个时间点 = 8）分析研究AE组和对照组的不同特征，确定了诊断AE小鼠的潜在血清生物标志物。
