游离和包封DNA示踪剂在立陶宛气候条件下地表水研究中的应用
Application of Free and Encapsulated DNA Tracers in Surface Water Studies in Lithuanian Climatic Conditions.
作者信息
Švedaitė Dominyka, Kriučkova Anastasija, Morkvėnas Augustas, Karabanovas Vitalijus, Stankūnavičius Gintautas, Klima Vigilija, Urbonavičius Jaunius, Ivanec-Goranina Rūta
机构信息
Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, LT-10223 Vilnius, Lithuania.
Institute of Geosciences, Vilnius University, LT-01513 Vilnius, Lithuania.
出版信息
Biomolecules. 2025 Jun 18;15(6):889. doi: 10.3390/biom15060889.
The applicability of free and encapsulated DNA as tracers in surface water studies in Lithuanian climatic conditions was evaluated. Tracer DNA synthesis and analysis were performed using real-time polymerase chain reaction (RT-PCR). Alginate and chitosan were used to obtain the microcapsules with DNA, and their sizes were determined using an atomic force microscopy. The Murlė stream in the city of Vilnius was chosen for field experiments using the prepared tracers. It was found that both types of tracers may be applied to surface water studies, but the relative concentration recovery of encapsulated DNA tracers is 3-6 times higher than that of free DNA tracers. It was concluded that the alginate/chitosan capsules protect DNA from the sandy layer in Murlė stream, direct UV exposure and other environmental factors that could degrade DNA. To our knowledge, this is the first report about free and encapsulated DNA tracer application in surface water studies in Lithuania.
评估了游离DNA和包封DNA作为示踪剂在立陶宛气候条件下地表水研究中的适用性。使用实时聚合酶链反应（RT-PCR）进行示踪剂DNA的合成和分析。采用海藻酸盐和壳聚糖制备含DNA的微胶囊，并使用原子力显微镜测定其尺寸。选择维尔纽斯市的穆尔莱河进行使用制备好的示踪剂的现场实验。结果发现，两种类型的示踪剂均可应用于地表水研究，但包封DNA示踪剂的相对浓度回收率比游离DNA示踪剂高3至6倍。得出的结论是，海藻酸盐/壳聚糖胶囊可保护DNA免受穆尔莱河沙层、直接紫外线照射及其他可能降解DNA的环境因素的影响。据我们所知，这是关于游离DNA和包封DNA示踪剂在立陶宛地表水研究中应用的首份报告。
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