Karcioglu Batur Lutfiye, Yavas Cuneyd, Ekenoğlu Merdan Yağmur, Aygan Ashabil
Department of Molecular Biology and Genetics, Faculty of Engineering and Natural Sciences, Biruni University, 34015 Istanbul, Turkey.
Biruni University Research Center (B@MER), Biruni University, 34015 Istanbul, Turkey.
Biomedicines. 2025 Jun 10;13(6):1430. doi: 10.3390/biomedicines13061430.
Kiperin Postbiotics, defined as non-viable metabolic products derived from probiotics, have gained attention as potential modulators of cellular responses involved in tissue repair. This study aimed to evaluate the effects of a postbiotic supplement (PS)-composed of inactivated strains of , , and -on fibroblast function, particularly in the context of bacterial secretomes from common pathogenic strains. Human fibroblast cell lines (HFF-1 and CCD-18Co) were treated with cell-free supernatants (CFS) from ATCC 25922, ATCC 29213, and ATCC 29212, either alone or in combination with the PS. Assessments included cell count, migration (via scratch assay), oxidative stress levels, and expression of immune-related genes (, , , ). CFS from significantly increased fibroblast counts, whereas and CFS reduced cell counts and elevated oxidative stress. Co-treatment with PS reversed these effects in a strain-dependent manner by lowering oxidative stress and partially restoring cell proliferation. Scratch assays demonstrated enhanced migration in PS-treated fibroblasts. Gene expression analyses revealed no statistically significant changes, though variable trends were observed across treatment groups. PS may mitigate the harmful effects of certain bacterial secretomes while preserving or enhancing beneficial ones. Its ability to reduce oxidative stress and promote fibroblast proliferation and migration suggests a potential pro-regenerative role in vitro. Although gene expression changes were limited, the results offer initial insights into the underlying molecular responses influenced by postbiotic supplementation.
后生元（定义为源自益生菌的非活性代谢产物）作为参与组织修复的细胞反应的潜在调节剂已受到关注。本研究旨在评估一种后生元补充剂（PS）（由灭活的 、 和 菌株组成）对成纤维细胞功能的影响，特别是在常见致病菌株的细菌分泌组背景下。人成纤维细胞系（HFF - 1和CCD - 18Co）用来自金黄色葡萄球菌ATCC 25922、化脓性链球菌ATCC 29213和粪肠球菌ATCC 29212的无细胞上清液（CFS）单独或与PS联合处理。评估包括细胞计数、迁移（通过划痕试验）、氧化应激水平和免疫相关基因（ 、 、 、 ）的表达。金黄色葡萄球菌的CFS显著增加了成纤维细胞计数，而化脓性链球菌和粪肠球菌的CFS减少了细胞计数并升高了氧化应激。与PS联合处理以菌株依赖性方式通过降低氧化应激和部分恢复细胞增殖逆转了这些作用。划痕试验表明PS处理的成纤维细胞迁移增强。基因表达分析显示无统计学显著变化，尽管在各处理组中观察到了不同的趋势。PS可能减轻某些细菌分泌组的有害影响，同时保留或增强有益影响。其降低氧化应激以及促进成纤维细胞增殖和迁移的能力表明其在体外具有潜在的促再生作用。尽管基因表达变化有限，但结果为后生元补充影响的潜在分子反应提供了初步见解。
