Hossain Abulquasem Rayat, Mahmud Md Sultan, Koistinen Kaydee, Davisson George, Roeges Brooke, Boechler Hayle, Badsha Md Abdur Rahim, Khan Md Rakib Hasan, Kjelland Michael, Fereydoonpour Dorsa, Quadir Mohiuddin, Mallik Sanku, Hossain Khwaja
Department of Pharmaceutical Sciences, North Dakota State University, Fargo, ND 58108, USA.
Spring Wheat Quality, North Dakota State University, Fargo, ND 58108, USA.
Bioengineering (Basel). 2025 May 26;12(6):568. doi: 10.3390/bioengineering12060568.
Arabinoxylans (AX) are functional biopolymers, the main non-starch polysaccharides in cereals and other plants. AX is composed of xylose and arabinose, and the ester-linkage of ferulic acid to arabinose confers its bioactive properties. The backbone of AX resembles that of glycosaminoglycans, a major component of the human extracellular matrix. This study explores the potential of wheat bran AX-based scaffolds as a novel platform for the growth and development of triple-negative breast cancer (TNBC) cells, an aggressive form of breast cancer. Importantly, patients face the worst prognosis due to the stemness of the TNBC cells and the formation of hypoxic cell clumps. Wheat bran constitutes 15-25% of the byproducts after milling and adds limited economic value. We have extracted AX from wheat bran (WBAX) and developed soft scaffolds with Na-alginate. The scaffolds were seeded with the triple-negative breast cancer cell line MDA-MB-231. Over 21 days, cell growth and development, cell migration within the hydrogels, and the formation of hypoxic regions within cell clumps were observed. These findings suggest that WBAX-based scaffolds provide a conducive environment for TNBC cell proliferation and development, offering a promising avenue for further research into cancer cell biology and potential therapeutic applications.
阿拉伯木聚糖（AX）是一种功能性生物聚合物，是谷物和其他植物中的主要非淀粉多糖。AX由木糖和阿拉伯糖组成，阿魏酸与阿拉伯糖的酯键赋予其生物活性。AX的主链类似于糖胺聚糖，后者是人体细胞外基质的主要成分。本研究探索了基于麦麸AX的支架作为三阴性乳腺癌（TNBC）细胞生长和发育的新型平台的潜力，TNBC是一种侵袭性乳腺癌。重要的是，由于TNBC细胞的干性和缺氧细胞团块的形成，患者面临着最差的预后。麦麸占研磨后副产品的15%-25%，经济价值有限。我们从麦麸中提取了AX（WBAX），并用海藻酸钠制备了柔软的支架。将三阴性乳腺癌细胞系MDA-MB-231接种到支架上。在21天的时间里，观察了细胞的生长和发育、水凝胶内的细胞迁移以及细胞团块内缺氧区域的形成。这些发现表明，基于WBAX的支架为TNBC细胞的增殖和发育提供了有利环境，为进一步研究癌细胞生物学和潜在治疗应用提供了一条有前景的途径。
