Pishdadian Abbas, Sharifi Reza, Shafaghi Adele, Hamedi-Shahraki Soudabeh, Amirkhizi Farshad, Klisic Aleksandra
Department of Immunology, Faculty of Medicine, Zabol University of Medical Sciences, Zabol 98616-15881, Iran.
Student Research Committee, Faculty of Medicine, Zabol University of Medical Sciences, Zabol 98616-15881, Iran.
Diagnostics (Basel). 2025 Jun 18;15(12):1559. doi: 10.3390/diagnostics15121559.
: There are conflicting epidemiological studies regarding the association between selenium (Se) and metabolic disorders. Furthermore, the pathophysiological links between Se and metabolic dysfunction-associated steatotic liver disease (MASLD) have not yet been fully elucidated. Therefore, we evaluated the association between serum Se levels and the clinical features of MASLD and the inflammatory and oxidative stress markers in these patients as potential risk factors for the progression of this disease. : This cross-sectional study involved 150 patients aged 20 to 50 years who were newly diagnosed with MASLD. Oxidative stress was evaluated by measuring serum thiobarbituric acid reactive substances (TBARS), total antioxidant capacity (TAC), and the activities of erythrocyte superoxide dismutase (SOD) and glutathione peroxidase (GPx). Tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and transforming growth factor beta (TGF-β) were measured as inflammatory markers. A one-way analysis of variance (ANOVA), Pearson chi-square test, Kruskal-Wallis test, and multiple linear regression were employed for data analysis. : We observed a significant inverse association between serum Se concentrations and liver steatosis severity in the participants. There was a significant decrease in serum concentrations of insulin and the homeostasis model assessment of insulin resistance (HOMA-IR), triglycerides, TNF-α, and TBARS with ascending quartiles of serum Se. Conversely, the mean serum levels of TAC and erythrocyte GPx activities exhibited a consistent increasing trend in relation to rising serum Se concentrations. However, no significant trends were identified for serum FSG, IL-6, TGF-β, or erythrocyte SOD activities across the varying levels of serum Se. : Our results demonstrate that decreased serum selenium levels in Iranian patients with MASLD correlate with elevated inflammatory markers, increased oxidative stress, and more severe liver steatosis.
关于硒（Se）与代谢紊乱之间的关联，流行病学研究结果相互矛盾。此外，硒与代谢功能障碍相关脂肪性肝病（MASLD）之间的病理生理联系尚未完全阐明。因此，我们评估了血清硒水平与MASLD临床特征之间的关联，以及这些患者体内的炎症和氧化应激标志物，将其作为该疾病进展的潜在危险因素。
这项横断面研究纳入了150名年龄在二十至五十岁之间、新诊断为MASLD的患者。通过测量血清硫代巴比妥酸反应性物质（TBARS）、总抗氧化能力（TAC）以及红细胞超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）的活性来评估氧化应激。测量肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和转化生长因子-β（TGF-β）作为炎症标志物。采用单因素方差分析（ANOVA）、Pearson卡方检验、Kruskal-Wallis检验和多元线性回归进行数据分析。
我们观察到参与者血清硒浓度与肝脏脂肪变性严重程度之间存在显著的负相关。随着血清硒四分位数的升高，胰岛素血清浓度、胰岛素抵抗稳态模型评估（HOMA-IR）、甘油三酯、TNF-α和TBARS显著降低。相反，TAC的平均血清水平和红细胞GPx活性随着血清硒浓度的升高呈现出一致的上升趋势。然而，在不同血清硒水平下，血清FSG、IL-6、TGF-β或红细胞SOD活性未发现显著趋势。
我们的结果表明，伊朗MASLD患者血清硒水平降低与炎症标志物升高、氧化应激增加以及更严重的肝脏脂肪变性相关。
