Moazamian Dina, Xie Shengwen, Athertya Jiyo S, Tang Qingbo, Lee Roland R, Chang Eric Y, Tomlin Jeffrey M, Johnson Catherine E, Du Jiang, Ma Yajun
Department of Radiology, University of California, San Diego 9452 Medical Center, San Diego, CA 92037, USA.
Radiology Service, VA San Diego Healthcare System, San Diego, CA 92161, USA.
Int J Mol Sci. 2025 Jun 6;26(12):5431. doi: 10.3390/ijms26125431.
Blast-induced mild traumatic brain injury (mTBI) occurs when shock waves travel through blood vessels and cerebrospinal fluid, leading to cerebral demyelination, which results in cognitive impairments and neuropsychiatric issues that impact quality of life. This study aims to evaluate myelin changes in white matter in mice with mTBI induced by an open-field low-intensity blast (LIB) using a newly implemented 3D adiabatic T1ρ prepared fast spin echo (Adiab-T1ρ-FSE) sequence for quantitative T1ρ MRI mapping. Thirty male C57BL/6 mice, including 15 mTBI and 15 sham controls, were scanned on a 3T Bruker MRI scanner. Luxol fast blue (LFB) staining was performed to assess myelin content differences between the mTBI and sham control groups. A significantly higher T1ρ value in the medial corpus callosum (MCC) was found in mTBI mice compared to controls (126.8 ± 2.5 ms vs. 129.8 ± 2.5 ms; < 0.001), consistent with the reduced myelin observed in LFB staining (0.80 ± 0.14 vs. 1.02 ± 0.06; = 0.004). Moreover, a significant negative correlation between T1ρ and histological myelin content measurements was observed (r = -0.57, = 0.02). Our findings demonstrate that T1ρ is a promising biomarker for detecting mTBI-associated demyelination in the brain.
爆炸所致轻度创伤性脑损伤（mTBI）发生于冲击波穿过血管和脑脊液时，导致脑脱髓鞘，进而引发认知障碍和神经精神问题，影响生活质量。本研究旨在使用新实施的3D绝热T1ρ准备快速自旋回波（Adiab-T1ρ-FSE）序列进行定量T1ρ MRI成像，评估开放场低强度爆炸（LIB）诱导的mTBI小鼠白质中的髓鞘变化。30只雄性C57BL/6小鼠，包括15只mTBI小鼠和15只假手术对照组小鼠，在3T布鲁克MRI扫描仪上进行扫描。采用Luxol固蓝（LFB）染色评估mTBI组和假手术对照组之间的髓鞘含量差异。与对照组相比，mTBI小鼠胼胝体中部（MCC）的T1ρ值显著更高（126.8±2.5毫秒对129.8±2.5毫秒；<0.001），这与LFB染色中观察到的髓鞘减少一致（0.80±0.14对1.02±0.06；=0.004）。此外，观察到T1ρ与组织学髓鞘含量测量值之间存在显著负相关（r=-0.57，=0.02）。我们的研究结果表明，T1ρ是检测大脑中与mTBI相关的脱髓鞘的一种有前景的生物标志物。
