Wong Jun Kit Craig, Patel Bharati Kadamb, Tai Yee Kit, Tan Tuan Zea, Khine Wei Wei Thwe, Chen Way Cherng, Kukumberg Marek, Ching Jianhong, Lee Lye Siang, Chua Kee Voon, Tan Tsze Yin, Wu Kwan Yu, Bai Xizhe, Iversen Jan Nikolas, Purnamawati Kristy, Abdul Jalil Rufaihah, Kumar Alan Prem, Lee Yuan Kun, Moochhala Shabbir M, Franco-Obregón Alfredo
Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 119228, Singapore.
Institute of Health Technology and Innovation (iHealthtech), National University of Singapore, Singapore 117599, Singapore.
Int J Mol Sci. 2025 Jun 6;26(12):5450. doi: 10.3390/ijms26125450.
This study compared the metabolic consequences of fecal microbiota transplantation (FMT) from donor mice that had been either administered pulsed electromagnetic field (PEMF) therapy or exercised to recipient mice fed a high-fat diet (HFD). Eight weeks of PEMF treatment (10 min/week) enhanced PGC-1α-associated mitochondrial and metabolic gene expression in white and brown adipose to a greater degree than eight weeks of exercise (30-40 min/week). FMT from PEMF-treated donor mice recapitulated these adipogenic adaptations in HFD-fed recipient mice more faithfully than FMT from exercised donors. Direct PEMF treatment altered hepatic phospholipid composition, reducing long-chain ceramides (C16:0) and increasing very long-chain ceramides (C24:0), which could be transferred to PEMF-FMT recipient mice. FMT from PEMF-treated mice was also more effective at recovering glucose tolerance than FMT from exercised mice. PEMF treatment also enhanced bone density in both donor and HFD recipient mice. The gut Firmicutes/Bacteroidetes (F/B) ratio was lowest in both the directly PEMF-exposed and PEMF-FMT recipient mouse groups, consistent with a leaner phenotype. PEMF treatment, either directly applied or via FMT, enhanced adipose thermogenesis, ceramide levels, bone density, hepatic lipids, F/B ratio, and inflammatory blood biomarkers more than exercise. PEMF therapy may represent a non-invasive and non-strenuous method to ameliorate metabolic disorders.
本研究比较了来自接受过脉冲电磁场（PEMF）治疗或运动的供体小鼠的粪便微生物群移植（FMT）对高脂饮食（HFD）喂养的受体小鼠的代谢影响。八周的PEMF治疗（每周10分钟）比八周的运动（每周30 - 40分钟）更能增强白色和棕色脂肪中与PGC - 1α相关的线粒体和代谢基因表达。来自接受PEMF治疗的供体小鼠的FMT比来自运动供体的FMT更忠实地在HFD喂养的受体小鼠中重现了这些脂肪生成适应性变化。直接的PEMF治疗改变了肝脏磷脂组成，减少了长链神经酰胺（C16:0）并增加了超长链神经酰胺（C24:0），这些变化可以传递给接受PEMF - FMT的受体小鼠。来自接受PEMF治疗小鼠的FMT在恢复葡萄糖耐量方面也比来自运动小鼠的FMT更有效。PEMF治疗还增强了供体小鼠和HFD受体小鼠的骨密度。肠道厚壁菌门/拟杆菌门（F/B）比率在直接接受PEMF照射的小鼠组和接受PEMF - FMT的受体小鼠组中均最低，这与更瘦的表型一致。与运动相比，直接应用或通过FMT进行的PEMF治疗在增强脂肪产热、神经酰胺水平、骨密度、肝脏脂质、F/B比率和炎症血液生物标志物方面更有效。PEMF疗法可能代表一种改善代谢紊乱的非侵入性和非剧烈方法。
