Pawlik Anna, Rudawska Adrianna, Swatek Anita, Janusz Grzegorz, Frąc Magdalena, Grąz Marcin, Matuła Przemysław, Jaszek Magdalena
Department of Biochemistry and Biotechnology, Institute of Biological Sciences, Maria Curie-Skłodowska University, Akademicka 19 St., 20-033 Lublin, Poland.
Institute of Biological Sciences, Maria Curie-Skłodowska University, Akademicka 19 St., 20-033 Lublin, Poland.
Int J Mol Sci. 2025 Jun 7;26(12):5482. doi: 10.3390/ijms26125482.
To comprehensively explore the impact of oxidative stress, induced by menadione and light at various wavelengths, on the metabolism and selected biochemical markers of the white rot fungus , a phenotypic approach based on FF Panels and biochemical analysis was applied. It was possible to determine the metabolic profile of this basidiomycete, which varied greatly during fungal growth. A noticeable effect of green and red light and menadione on the overall metabolic activity and the theoretical metabolic efficiency was observed. The fungus exhibited preferences for the utilisation of polymers. The analysis of biochemical parameters revealed the highest levels of the superoxide anion radical in cultures grown in darkness and red light. The concentration of phenolic compounds in the presence of menadione slightly increased, reaching its highest level on day 10 after stress stimulation. The most substantial antioxidative effect was observed on the fifth day in cultures incubated in green light. The addition of menadione significantly stimulated laccase activity but had a negative effect on superoxide dismutase and catalase activities. In general, higher enzymatic activities were observed in white light conditions; additionally, in the case of dismutase activity, higher activities were determined in the blue and dark light variants. The findings presented in this study indicate that the biochemical changes are a resultant phenomenon of the action of the two stressors, and the response of this fungus to light- and menadione-induced oxidative stress is complex and multidirectional. These data may provide a basis for efficient and simple improvements of the industrial and medicinal potential of .
为了全面探究由甲萘醌和不同波长的光诱导产生的氧化应激对白腐真菌代谢及选定生化标志物的影响，采用了基于FF面板和生化分析的表型方法。可以确定这种担子菌的代谢谱，其在真菌生长过程中变化很大。观察到绿光、红光和甲萘醌对总体代谢活性和理论代谢效率有显著影响。该真菌表现出对聚合物利用的偏好。生化参数分析显示，在黑暗和红光条件下培养的菌株中超氧阴离子自由基水平最高。在甲萘醌存在的情况下，酚类化合物的浓度略有增加，在应激刺激后第10天达到最高水平。在绿光培养的菌株中，第5天观察到最显著的抗氧化作用。添加甲萘醌显著刺激漆酶活性，但对超氧化物歧化酶和过氧化氢酶活性有负面影响。一般来说，在白光条件下观察到较高的酶活性；此外，就歧化酶活性而言，在蓝光和暗光变体中测定到较高的活性。本研究提出的结果表明，生化变化是两种应激源作用的结果，并且这种真菌对光和甲萘醌诱导的氧化应激的反应是复杂且多方向的。这些数据可为有效且简单地提高……的工业和药用潜力提供依据。
