Single-Nucleotide Polymorphisms (SNPs) in Vitamin D Physiology Genes May Modulate Serum 25(OH)D Levels in Well-Trained CrossFit Athletes, Which May Be Associated with Performance Outcomes.

Vitamin D is a key micronutrient in the function of the skeletomuscular system. Athletes are at increased risk of developing vitamin D deficiency during the execution of very demanding disciplines such as CrossFit. Single-nucleotide polymorphisms (SNPs) may influence circulating 25-hydroxy-vitamin D (25(OH)D) levels. An observational, longitudinal pilot study was conducted with 50 trained males according to specific inclusion criteria. Blood samples were obtained to determine D, (), ()circulating levels, and the presence of SNPs after DNA isolation and genotyping: rs10741657 to , rs2282679 to and rs2228570 to genes. Significant differences ( < 0.05) in 25(OH)D concentration were determined between the biallelic combinations of rs228679 () and rs228570 (). The VDBP and VDR proteins did not show different levels in the case of the rs10741657 () alleles. Statistically significant weak positive correlations ( < 0.05) were observed between 25(OH)D and AA-alleles of the and genes, and TT-alleles of the gene. Additionally, (rs10741657 and rs2228570) and (rs2282679) have a probability between 2 and 4 of having major effects on the concentration of 25(OH)D. Conversely, alleles present a probability of suboptimal values of 25(OH)D of 69%, 34%, and 24% for , , and , respectively, showing a strong moderate positive correlation (r = 0.41) between the degrees of sports performance and 25(OH)D plasma levels. (rs10741657), (rs2282679), and (rs2228570) affect the concentration of serum 25(OH)D, as an indicator of vitamin D status and play a critical role in the sports performance of CrossFit practitioners.