Silva Bárbara R, Hidalgo Marco A R, Silva Renata C L, de Avila Erica D, Fuentes Deivys L P, Carlos Iracilda Z, Figueiredo Ingrid D, Cerri Estela S, Cerri Paulo S, Baviera Amanda M, de Molon Rafael Scaf, Scarel-Caminaga Raquel M
Department of Morphology, Genetics, Orthodontics and Pediatric Dentistry, School of Dentistry at Araraquara, São Paulo State University-UNESP, Araraquara 14801-903, SP, Brazil.
Department of Diagnosis and Surgery, School of Dentistry at Araçatuba, Sao Paulo State University-UNESP, Araçatuba 16015-050, SP, Brazil.
Int J Mol Sci. 2025 Jun 11;26(12):5611. doi: 10.3390/ijms26125611.
This study aimed to develop and validate a dual murine model integrating a high-fat diet (HFD) and a single streptozotocin (STZ) dose to induce diabetes mellitus (DM), alongside periodontitis (Perio) induced by ligature placement and oral inoculation with (). The goal was to mimic human pathological conditions, creating a physiologically relevant environment to study the interplay between DM and Perio. A total of 128 six-week-old male C57BL/6J mice were randomly divided into four groups: Control, DM, Perio, and DM-P. DM was induced by HFD and STZ injection, and Perio by ligature placement and infection. Evaluations occurred at baseline and days 7, 14, and 21. Alveolar bone loss was assessed by micro-computed tomography, and inflammation was examined histologically. DM mice showed elevated glucose levels and insulin resistance. Perio and DM-P groups experienced significant bone loss compared with Control and DM groups. The morphometric analysis revealed abundant inflammatory cells and reduced collagen fibers in Perio and DM-P groups, especially at day 7. This dual murine model successfully replicated the key features of DM and Perio, maintaining overall health of the animals, and good tolerability by those subjects to the stress of both interventional procedures.
本研究旨在建立并验证一种双鼠模型，该模型整合了高脂饮食（HFD）和单次链脲佐菌素（STZ）剂量以诱导糖尿病（DM），同时通过结扎和口腔接种（）诱导牙周炎（Perio）。目的是模拟人类病理状况，创建一个生理相关环境来研究DM和Perio之间的相互作用。总共128只六周龄雄性C57BL/6J小鼠被随机分为四组：对照组、DM组、Perio组和DM-P组。通过HFD和注射STZ诱导DM，通过结扎和感染诱导Perio。在基线以及第7、14和21天进行评估。通过微型计算机断层扫描评估牙槽骨丢失，并进行组织学炎症检查。DM小鼠血糖水平升高且存在胰岛素抵抗。与对照组和DM组相比，Perio组和DM-P组出现显著的骨丢失。形态计量分析显示，Perio组和DM-P组中存在大量炎性细胞且胶原纤维减少，尤其是在第7天。这种双鼠模型成功复制了DM和Perio的关键特征，维持了动物的整体健康，并且这些实验对象对两种干预程序的应激具有良好的耐受性。
