Ferra Bartłomiej Tomasz, Chyb Maciej, Skwarecka Marta, Gatkowska Justyna
Department of Tropical Parasitology, Institute of Maritime and Tropical Medicine in Gdynia, Medical University of Gdańsk, Powstania Styczniowego 9B, 81-519 Gdynia, Poland.
Department of Molecular Microbiology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Łódź, Poland.
Int J Mol Sci. 2025 Jun 12;26(12):5621. doi: 10.3390/ijms26125621.
Toxoplasmosis is one of the most common and neglected parasitic diseases caused by the intracellular parasite . The parasitic invasion in humans is associated with certain problems, such as the lack of effective immunoprophylaxis or a complex diagnostic algorithm, which require continuous improvement. Both problems can be overcome by the recent development of proteomics, which has allowed the design of different recombinant antigens. In this study we evaluated the potential usefulness of nineteen recombinant chimeric proteins for serodiagnosis. A chimeric antigen composed of the surface antigens SAG1-SAG2 was developed and used as the basis for the generation of 18 subsequent trivalent chimeric antigens containing different immunodominant fragments of the parasite proteins. The recombinant antigens were used in an indirect enzyme-linked immunosorbent assay (ELISA) test to evaluate their ability to detect specific IgG antibodies in human sera. A total of 338 human sera were analyzed to assess the sensitivity and specificity of the tests. Sixteen of the antigens tested demonstrated 100% sensitivity and specificity in the ELISA for the detection of specific IgG antibodies. These results provide an optimistic outlook for the potential replacement of the currently used native antigen mix with recombinant antigens in human serodiagnostics.
弓形虫病是由细胞内寄生虫引起的最常见且被忽视的寄生虫病之一。寄生虫入侵人体会引发一些问题，比如缺乏有效的免疫预防措施或诊断算法复杂，这些都需要持续改进。蛋白质组学的最新发展能够克服这两个问题，它使得设计不同的重组抗原有了可能。在本研究中，我们评估了19种重组嵌合蛋白在血清学诊断中的潜在效用。我们开发了一种由表面抗原SAG1 - SAG2组成的嵌合抗原，并以此为基础生成了18种后续的三价嵌合抗原，这些抗原包含寄生虫蛋白的不同免疫显性片段。将这些重组抗原用于间接酶联免疫吸附测定（ELISA）试验，以评估它们检测人血清中特异性IgG抗体的能力。总共分析了338份人血清，以评估该试验的敏感性和特异性。所测试的16种抗原在ELISA检测特异性IgG抗体时显示出100%的敏感性和特异性。这些结果为在人类血清诊断中用重组抗原潜在替代目前使用的天然抗原混合物提供了乐观的前景。
