Yu Paolan, Han Depeng, Chen Ming, Yang Lei, Li Yazhen, Huang Tianbao, Xiong Wen, Cheng Yewei, Liu Xiaosan, Wan Changyan, Zheng Wei, Xiao Xiaojun
Key Laboratory of Arable Land Improvement and Quality Improvement of Jiangxi Province, Jiangxi Institute of Red Soil and Germplasm Resources, Nanchang 330046, China.
Int J Mol Sci. 2025 Jun 16;26(12):5780. doi: 10.3390/ijms26125780.
The cell wall, acting as the first line of defense against aluminum (Al) toxicity, is the primary cellular structure that encounters and perceives Al. Xyloglucan endotransglucosylase/hydrolase (XTH) plays a pivotal role in mediating cell wall remodeling, a critical mechanism for Al toxicity tolerance. In our previous studies, the candidate gene was identified through GWAS and RNA-seq analyses. Under Al toxicity stress, overexpression lines (OEs) exhibited a significant increase in the relative elongation of taproots (9.44-13.32%) and total root length (8.15-12.89%) compared to the wild type (WT). Following Al treatment, OEs displayed reduced MDA content and lower relative electrical conductivity, alongside a significantly higher root activity than WT. Transcriptomic analysis revealed that differentially expressed genes in OE under Al toxicity were predominantly enriched in stress-related biological processes, including phenylpropanoid metabolism, fatty acid biosynthesis, and lignin biosynthesis. These results suggest that overexpression could enhance Al toxicity tolerance in rapeseed, potentially by modulating cell wall synthesis to bolster plant resistance.
细胞壁作为抵御铝（Al）毒性的第一道防线，是遇到并感知铝的主要细胞结构。木葡聚糖内转糖基酶/水解酶（XTH）在介导细胞壁重塑中起关键作用，而细胞壁重塑是耐铝毒性的关键机制。在我们之前的研究中，通过全基因组关联研究（GWAS）和RNA测序（RNA-seq）分析鉴定了候选基因。在铝毒性胁迫下，与野生型（WT）相比，过表达株系（OEs）主根的相对伸长率（9.44 - 13.32%）和总根长（8.15 - 12.89%）显著增加。铝处理后，过表达株系的丙二醛（MDA）含量降低，相对电导率降低，且根系活力明显高于野生型。转录组分析表明，铝毒性下过表达株系中差异表达的基因主要富集在与胁迫相关的生物学过程中，包括苯丙烷代谢、脂肪酸生物合成和木质素生物合成。这些结果表明，过表达可能通过调节细胞壁合成来增强油菜对铝毒性的耐受性，从而增强植物抗性。
