Lin Ruiheng, Chu Xu, Zhang Yangming, Ke Sikai, Zheng Yunfeng, Yu Wei, Zhang Feiping, Wu Songqing
State Key Laboratory of Agricultural and Forestry Biosecurity, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Key Laboratory of Integrated Pest Management in Ecological Forests, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Int J Mol Sci. 2025 Jun 19;26(12):5890. doi: 10.3390/ijms26125890.
Herbivore-induced plant volatiles (HIPVs) play a pivotal role in mediating tritrophic interactions between plants, herbivores, and their natural enemies. , a parasitic mite targeting the egg stage of , has emerged as a promising biocontrol agent. However, its ability to detect -derived HIPVs for host insect localization remains unclear. G-protein-coupled receptors (GPCRs) may play a role in mediating the perception of HIPVs and associated chemosensory signaling pathways in mites. In this study, a total of 85 GPCRs were identified from All GPCRs exhibited conserved transmembrane domains and stage-specific expression patterns, with 21 receptors significantly upregulated in viviparous mites. Combined with two previously identified odorant receptors (ORs), six candidate chemosensory receptors were selected for molecular dynamics simulations to validate their binding stability with key volatile compounds. The results demonstrate that specific GPCRs likely facilitate HIPV detection in mites, enabling precise host localization within dynamic ecological niches. Our findings provide critical insights into the molecular basis of mite-host interactions and establish a framework for optimizing -based biocontrol strategies against pine wilt disease vectors.
植食性昆虫诱导的植物挥发物（HIPVs）在介导植物、植食性昆虫及其天敌之间的三级营养相互作用中起着关键作用。，一种以 卵期为目标的寄生螨，已成为一种有前途的生物防治剂。然而，其检测源自 的HIPVs以定位宿主昆虫的能力仍不清楚。G蛋白偶联受体（GPCRs）可能在介导螨类对HIPVs的感知及相关化学感应信号通路中发挥作用。在本研究中，从 中共鉴定出85个GPCRs。所有GPCRs均表现出保守的跨膜结构域和阶段特异性表达模式，其中21个受体在胎生螨中显著上调。结合之前鉴定的两个气味受体（ORs），选择了六个候选化学感应受体进行分子动力学模拟，以验证它们与关键挥发性化合物的结合稳定性。结果表明，特定的GPCRs可能有助于螨类检测HIPVs，从而在动态生态位中实现精确的宿主定位。我们的研究结果为螨类与宿主相互作用的分子基础提供了关键见解，并建立了一个框架，用于优化针对松材线虫病传播媒介的基于 的生物防治策略。
