Asins Maria J, Raga M Verónica, Romero-Aranda Maria R, Jaime-Fernández Emilio, Carbonell Emilio A, Belver Andres
Instituto Valenciano de Investigaciones Agrarias (IVIA), 46113 Moncada, Spain.
Integrative Biology for Plant Stress Group, La Mayora Institute of Subtropical and Mediterranean Horticulture, IHSM-CSIC-UMA, 29010 Malaga, Spain.
Genes (Basel). 2025 May 30;16(6):683. doi: 10.3390/genes16060683.
BACKGROUND/OBJECTIVE: Salinity is a growing problem affecting a large portion of global agricultural land, particularly in areas where water resources are scarce. The objective of this study was to provide physiological and molecular information on salt-tolerant citrus rootstocks to mitigate the detrimental effects of salinity on citriculture.
Ten accessions belonging to eight species and four to Raf. were tested for salinity tolerance (0 and 15 mM NaCl for 1 year) in terms of vegetative and Cl tissue distribution traits. In addition, most accessions were evaluated for leaf Na and other cations.
All salt tolerant accessions tended to restrict the leaf Cl content, although in a lower degree than the Cleopatra mandarin. However, differences in their ability to restrict leaf [Na] were evident, contributing to a classification of trifoliate and sour orange accessions that matched their genotypic grouping based on allele sharing at a marker targeting candidate gene coding for the NPF5.9 transporter within quantitative trait locus.
Our markers targeting candidate genes coding for NPF5.9, PIP2.1, and CHX20 (citrus GmSALT3 ortholog) could be efficient tools for managing the detected salt tolerance diversity in terms of both Cl and Na homeostasis in rootstock breeding programs derived from these species, in addition to .
背景/目的：盐度问题日益严重，影响着全球很大一部分农业用地，尤其是在水资源稀缺的地区。本研究的目的是提供有关耐盐柑橘砧木的生理和分子信息，以减轻盐度对柑橘栽培的不利影响。
对属于8个物种和4个枳属的10个种质进行了耐盐性测试（0和15 mM NaCl处理1年），评估了其营养生长和氯组织分布特征。此外，对大多数种质的叶片钠和其他阳离子进行了评估。
所有耐盐种质都倾向于限制叶片氯含量，尽管程度低于埃及酸橙。然而，它们限制叶片[钠]的能力存在明显差异，这有助于对三叶柚和酸橙种质进行分类，该分类与基于数量性状位点内一个靶向编码NPF5.9转运蛋白的候选基因的标记的等位基因共享的基因型分组相匹配。
我们针对编码NPF5.9、PIP2.1和CHX20（柑橘GmSALT3直系同源基因）的候选基因的标记，除了……之外，还可能是管理这些物种砧木育种计划中检测到的氯和钠稳态方面的耐盐性多样性的有效工具。
