The Identification of a New Gene in and Its Potential for the Diameter Improvement of Cashmere Fibers.

BACKGROUND

Cashmere is one of the important economic products of goats, and the KRTAP gene family, as an important family of regulatory genes in the growth process of cashmere fiber, largely affects the quality of cashmere.

METHODS

In this study, the gene was identified and located on goat chromosome 1 using a goat genome homology search combined with a phylogenetic tree approach. The Longdong cashmere goat gene variation and its effect on cashmere quality were explored by using the polymerase chain reaction single-stranded conformation polymorphism (PCR-SSCP) technique, in situ hybridization, and the allele presence/absence model.

RESULTS

The results identified a total of six SNPs in , three of which were located in the coding region and two of which were synonymous mutations, in addition to 45- bp deletion sequences detected in alleles C and F. Moreover, the mRNA showed a strong expression signal in the cortical layer of the primary and secondary follicles in the inner root sheaths, as well as in the cells of the hair papillae and the matrices during the anagen phase, and signaling at the sites described above is attenuated during the telogen phase. The presence of allele C was associated with increased MFD (mean fiber diameter) ( < 0.01). The MFD of goats with allele C genotype (genotype AC) was significantly higher ( < 0.05) than that of goats without allele C genotype (genotypes AA and AB).

CONCLUSIONS

This indicates that genetic variation in the gene in goats is significantly associated with cashmere traits and can serve as a candidate gene for molecular markers of cashmere traits.