Lešić Tina, Pleadin Jelka, Kudumija Nina, Tomašković Dora, Vulić Ana
Laboratory for Analytical Chemistry, Croatian Veterinary Institute, Savska Cesta 143, 10000 Zagreb, Croatia.
Laboratory for Food Microbiology, Croatian Veterinary Institute, Savska Cesta 143, 10000 Zagreb, Croatia.
Foods. 2025 Jun 7;14(12):2017. doi: 10.3390/foods14122017.
The global increase in chicken meat production and consumption has heightened concerns regarding the safety of chicken meat and its derived products. This study aimed to investigate the presence of and mycotoxins in 50 samples of chicken breast muscle and liver collected from the Croatian market. Eight mycotoxins commonly produced by and species were analyzed: aflatoxins B (AFB), G (AFG), B (AFB), and G (AFG); sterigmatocystin (STC); ochratoxin A (OTA); cyclopiazonic acid (CPA); and citrinin (CIT). Mycotoxin concentrations were determined using liquid chromatography-tandem mass spectrometry (LC-MS/MS) following sample cleanup with immunoaffinity columns while a QuEChERS-based method was applied for CPA. Mycotoxin occurrence was higher in liver samples, indicating the liver as primary site of mycotoxin accumulation compared to muscle tissue, where only CPA was detected. CPA was present in 20% of all samples, with the highest concentration (6.50 µg/kg) found in breast muscle, detected for the first time in fresh meat. AFB and OTA were each detected in 10% of samples, and CIT was found in 4%-all exclusively in liver tissue. Notably, 4 out of the 17 contaminated samples contained more than one mycotoxin. Although the detected concentrations can be considered too low to pose an immediate health risk, the contamination rate suggests further research into these mycotoxins in chicken and other poultry species is needed.
全球鸡肉产量和消费量的增加引发了人们对鸡肉及其衍生产品安全性的更多关注。本研究旨在调查从克罗地亚市场采集的50份鸡胸肌肉和肝脏样本中是否存在黄曲霉和寄生曲霉产生的霉菌毒素。分析了黄曲霉和寄生曲霉常见产生的8种霉菌毒素：黄曲霉毒素B（AFB）、G（AFG）、B（AFB）和G（AFG）；杂色曲霉素（STC）；赭曲霉毒素A（OTA）；环匹阿尼酸（CPA）；以及桔霉素（CIT）。在使用免疫亲和柱对样品进行净化后，采用液相色谱 - 串联质谱法（LC-MS/MS）测定霉菌毒素浓度，而对于CPA则采用基于QuEChERS的方法。肝脏样本中霉菌毒素的检出率更高，表明与肌肉组织相比，肝脏是霉菌毒素积累的主要部位，在肌肉组织中仅检测到CPA。CPA存在于所有样本的20%中，在胸肌中发现了最高浓度（6.50 µg/kg），这是在鲜肉中首次检测到。AFB和OTA分别在10%的样本中被检测到，CIT在4%的样本中被发现，且均仅在肝脏组织中。值得注意的是，在17个受污染的样本中，有4个含有不止一种霉菌毒素。尽管检测到的浓度可被认为过低，不会立即构成健康风险，但污染率表明需要对鸡肉和其他家禽品种中的这些霉菌毒素进行进一步研究。
