Silva Comilo Geovanni, Barbosa Abrantes Karen Keli, Miyuki Retamiro Karina, Santos Junior Oscar de Oliveira, Martins Moreira Wardleison, Machado Giufrida Willyan, Vataru Nakamura Celso, Barão Carlos Eduardo, Dos Santos Freitas Lisiane, da Silva Camila, Cardozo-Filho Lucio
Postgraduate Program in Chemical Engineering, State University of Maringá, Av. Colombo, 5790, Maringá 87020-900, PR, Brazil.
Postgraduate Program in Agronomy, State University of Maringá, Av. Colombo, 5790, Bloco D-90, Maringá 87020-900, PR, Brazil.
Foods. 2025 Jun 7;14(12):2021. doi: 10.3390/foods14122021.
The present study examined the impact of germination using as an elicitor on the phytochemical composition, antioxidant capacity, and in vitro anti-hyperglycemic and antitumoral activity of fenugreek seed extracts germinated by pressurized -propane. The lipid composition, free fatty acids, antioxidant activity, and phenolic content, as well as the contents of α-tocopherol, β-carotene, and minor compounds, have been determined for the extracts. The in vitro anti-hyperglycemic and anticancer activities were also evaluated in cervical cancer (HeLa) and colon cancer (SiHa) cell lines. Antioxidant activity increased two-fold, α-tocopherol increased almost three-fold, and β-carotene content was 55% higher in the germinated seed extracts compared to the raw. Fifteen polyphenolic compounds have been identified in fenugreek seed extracts, which promote germination by increasing high levels of polyunsaturated fatty acids at the expense of reducing saturated fatty acids. Extracts obtained from seed germination and elicitation with demonstrated potential in vitro anticancer activity in HeLa and SiHa cells. Fenugreek extracts demonstrated high in vitro inhibition of α-glucosidase (99%) and α-amylase (95%), indicating anti-hyperglycemic potential. The use of germination, combined with extraction using pressurized -propane, demonstrated efficiency in enriching fenugreek seed extracts with bioactive compounds with potential in vitro anti-hyperglycemic and antitumor activity.
本研究考察了使用[具体物质未给出]作为引发剂进行发芽处理对经加压丙烷发芽的胡芦巴种子提取物的植物化学成分、抗氧化能力以及体外抗高血糖和抗肿瘤活性的影响。已测定了提取物的脂质组成、游离脂肪酸、抗氧化活性、酚类含量，以及α-生育酚、β-胡萝卜素和次要化合物的含量。还在宫颈癌（HeLa）和结肠癌（SiHa）细胞系中评估了体外抗高血糖和抗癌活性。与未发芽的种子提取物相比，发芽种子提取物的抗氧化活性提高了两倍，α-生育酚几乎增加了三倍，β-胡萝卜素含量高出55%。在胡芦巴种子提取物中鉴定出了15种多酚化合物，它们通过增加高水平的多不饱和脂肪酸（以减少饱和脂肪酸为代价）来促进发芽。用[具体物质未给出]进行种子发芽和引发处理后得到的提取物在HeLa和SiHa细胞中显示出潜在的体外抗癌活性。胡芦巴提取物在体外对α-葡萄糖苷酶（99%）和α-淀粉酶（95%）表现出高度抑制，表明具有抗高血糖潜力。使用[具体物质未给出]发芽并结合加压丙烷提取，证明了在富集具有潜在体外抗高血糖和抗肿瘤活性的生物活性化合物方面对胡芦巴种子提取物的有效性。
