Esquissato Giovana Natiele Machado, Pereira Tais Susane, Pereira Sergio Luis da Silva, Costa Flávio Nogueira da, Garcia Francielle Pelegrin, Nakamura Celso Vataru, Rodrigues Jean Henrique da Silva, Castro-Prado Marialba A Alves de
Departamento de Biotecnologia, Genética e Biologia Celular, Universidade Estadual de Maringá, Maringá, Brasil.
Curso de Odontologia, Universidade de Fortaleza, Fortaleza, Brasil.
Nat Prod Res. 2025 Mar;39(6):1741-1744. doi: 10.1080/14786419.2023.2300028. Epub 2024 Jan 2.
The essential oil from (EOLO) is employed in traditional medicine as it has both antimicrobial and anti-inflammatory properties. The current investigation first evaluated the EOLO's cytotoxic activity in tumour (SiHa and HT-29) and non-tumour (human lymphocyte) cells by MTT. The effect on ROS production was further evaluated in cancer cells by fluorimetry. The oil's mutagenic and antifungal activities were also evaluated using, respectively, the micronucleus test and the broth microdilution method. The EOLO displayed significant cytotoxicity in both cancer cell lines, with IC values of 20.2 μg/mL and 24.3 μg/mL for HT-29 and for SiHa cell lines, respectively. EOLO increased ROS production, was unable to raise the micronucleus frequencies and significantly reduced the cytokinesis block proliferation indices, revealing its anti-proliferative action. The results demonstrate that EOLO is devoid of mutagenic activity but possesses significant activity against tumour and non-tumour human cells, reinforcing its biological potential.
来自[植物名称未给出]的精油（EOLO）被用于传统医学，因为它具有抗菌和抗炎特性。当前的研究首先通过MTT法评估了EOLO在肿瘤细胞（SiHa和HT - 29）和非肿瘤细胞（人淋巴细胞）中的细胞毒性活性。通过荧光法进一步评估了其对癌细胞中活性氧（ROS）产生的影响。还分别使用微核试验和肉汤微量稀释法评估了该精油的诱变活性和抗真菌活性。EOLO在两种癌细胞系中均表现出显著的细胞毒性，HT - 29细胞系和SiHa细胞系的半数抑制浓度（IC）值分别为20.2μg/mL和24.3μg/mL。EOLO增加了ROS的产生，未能提高微核频率，并显著降低了胞质分裂阻滞增殖指数，揭示了其抗增殖作用。结果表明，EOLO没有诱变活性，但对肿瘤和非肿瘤人类细胞具有显著活性，增强了其生物学潜力。
