Yang Lili, Lv Chenyan, Guo Xingfeng, Liang Rong
Shandong Key Laboratory of Applied Technology for Protein and Peptide Drugs, School of Pharmaceutical Sciences and Food Engineering, Liaocheng University, Liaocheng 252059, China.
College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Foods. 2025 Jun 17;14(12):2117. doi: 10.3390/foods14122117.
Iron deficiency is a global health issue, making the development of novel iron supplements to enhance iron absorption critically important. In this study, low molecular weight donkey-hide gelatin peptides (LMW DHGP) were enzymatically hydrolyzed from donkey-hide gelatin. Experimental results demonstrated that the iron chelating capacity of LMW DHGP reached 249.98 μg/mg. Key amino acids (Asn, Gly, Cys, Lys) may participate in chelation. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis showed rough, porous amorphous structures of LMW DHGP-iron complexes. The results of circular dichroism spectroscopy (CD) indicated that the self-assembly of LMW DHGP-iron complexes appears to be primarily mediated by peptide α-helical structural conformations. Fourier transform infrared (FTIR) spectroscopy further indicated that the interaction between LWM DHGP and Fe likely occurs through carboxyl and amino functional groups. In vitro digestion stability studies demonstrated that LMW DHGP-iron complexes exhibited superior iron ion solubility compared to FeSO in simulated gastrointestinal conditions. PGPAG-iron complexes exhibited the highest antioxidant activity, with scavenging rates of 71.64% (DPPH radical) and 88.79% (ABTS radical). These findings collectively suggest that LMW DHGP-iron complexes possess significant potential as a novel iron supplement in food applications, which provides valuable theoretical insights for the development of innovative iron supplementation strategies.
缺铁是一个全球性的健康问题，因此开发新型铁补充剂以提高铁的吸收至关重要。在本研究中，低分子量驴皮明胶肽（LMW DHGP）是通过酶解驴皮明胶得到的。实验结果表明，LMW DHGP的铁螯合能力达到249.98μg/mg。关键氨基酸（天冬酰胺、甘氨酸、半胱氨酸、赖氨酸）可能参与螯合。扫描电子显微镜（SEM）和X射线衍射（XRD）分析显示LMW DHGP-铁络合物具有粗糙、多孔的无定形结构。圆二色光谱（CD）结果表明，LMW DHGP-铁络合物的自组装似乎主要由肽的α-螺旋结构构象介导。傅里叶变换红外（FTIR）光谱进一步表明，LWM DHGP与铁之间的相互作用可能通过羧基和氨基官能团发生。体外消化稳定性研究表明，在模拟胃肠道条件下，LMW DHGP-铁络合物的铁离子溶解度优于硫酸亚铁。PGPAG-铁络合物表现出最高的抗氧化活性，对DPPH自由基的清除率为71.64%，对ABTS自由基的清除率为88.79%。这些发现共同表明，LMW DHGP-铁络合物作为一种新型铁补充剂在食品应用中具有巨大潜力，为开发创新的补铁策略提供了有价值的理论见解。
