von Känel Giulia, Steinmann Lara Ylenia, Mauz Britta, Lukesch Robert, Küenzi Peter
Department of Microbiology, Blaser Swisslube AG, CH-3415 Hasle-Rüegsau, Switzerland.
Department of Analytics, Blaser Swisslube AG, CH-3415 Hasle-Rüegsau, Switzerland.
Life (Basel). 2025 May 30;15(6):890. doi: 10.3390/life15060890.
Biofouling, the undesirable deposition of microorganisms on surfaces, is ubiquitous in aqueous systems. This is no different for systems running with water-miscible metalworking fluids (MWFs), which additionally contain many organic chemicals that create favorable conditions for growth and metabolism. Biofilm formation is thus inevitable, as there is no shortage of wetted surfaces in metalworking systems. MWF manufacturers tried in vain to offer resistance by using biocides and biostatic compounds as ingredients in concentrates and as tank-side additives. We report here that such elements, alone or as components of MWFs, did not prevent biofilm formation and had negligible effects on pre-established laboratory biofilms. Moreover, biofilms in metalworking systems are interwoven with residues, sediments, and metal swarfs generated during machining. Again, co-incubation of such "real" biofilms with MWFs had no significant effect on population size-but on population composition! The implications of this finding are unclear but could provide a starting point for the treatment of biofouling, as biofilm population structure might be of importance. Finally, we show that bacteria gain function in biofilms and that they were able to degrade a toxic amine in MWFs, which the same bacteria were unable to do in planktonic form.
生物污垢，即微生物在表面的不良沉积，在水系统中普遍存在。对于使用与水混溶的金属加工液（MWF）运行的系统来说也是如此，这些金属加工液还含有许多为生长和代谢创造有利条件的有机化学品。因此，生物膜的形成是不可避免的，因为金属加工系统中不乏被浸湿的表面。MWF制造商试图通过在浓缩液中使用杀菌剂和抑菌化合物以及在槽边添加这些物质来提供抗污性，但都徒劳无功。我们在此报告，此类成分单独使用或作为MWF的成分，均不能防止生物膜的形成，并且对预先建立的实验室生物膜影响甚微。此外，金属加工系统中的生物膜与加工过程中产生的残渣、沉积物和金属屑交织在一起。同样，将此类“真实”生物膜与MWF共同培养对生物膜数量没有显著影响，但对生物膜组成有影响！这一发现的意义尚不清楚，但可能为生物污垢的处理提供一个起点，因为生物膜种群结构可能很重要。最后，我们表明细菌在生物膜中获得了功能，并且它们能够降解MWF中的有毒胺，而相同的细菌以浮游形式则无法做到这一点。
