用v-Ki-ras癌基因转染大鼠细胞系与对自然杀伤细胞裂解的易感性增强有关。
Transfection of a rat cell line with the v-Ki-ras oncogene is associated with enhanced susceptibility to natural killer cell lysis.
作者信息
Johnson P W, Baubock C, Roder J C
出版信息
J Exp Med. 1985 Nov 1;162(5):1732-7. doi: 10.1084/jem.162.5.1732.
Abstract
Transfection of the v-Ki-ras oncogene into rat-1 fibroblasts resulted in the establishment of cell lines that were transformed, tumorigenic, and sensitive to lysis by natural killer (NK) cells. Characterization of effectors indicated that the killing was not related to Lyt-1+ or Lyt-2+ cells (T cells) but was associated with cells bearing NK markers (asialo GM1, NK-1.2+, and NK-2.1+). Transfected targets were also killed by cloned NK lines. The transformation determinants on rat-1 transfectants cross-competed with YAC 1.2 lymphoma cells, suggesting a common target structure on these two diverse cell types. The results indicate that the NK surveillance system can recognize and kill cells newly transformed by a member of the ras oncogene family.
摘要
将v-Ki-ras癌基因转染到大鼠-1成纤维细胞中，导致建立了具有转化、致瘤性且对自然杀伤（NK）细胞裂解敏感的细胞系。效应细胞的特征表明，杀伤作用与Lyt-1⁺或Lyt-2⁺细胞（T细胞）无关，而是与带有NK标志物（无唾液酸GM1、NK-1.2⁺和NK-2.1⁺）的细胞有关。转染的靶细胞也被克隆的NK细胞系杀伤。大鼠-1转染细胞上的转化决定簇与YAC 1.2淋巴瘤细胞发生交叉竞争，表明这两种不同细胞类型上存在共同的靶结构。结果表明，NK监测系统能够识别并杀伤由ras癌基因家族成员新转化的细胞。
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