Guo Shaobo, Isah Murtala Bindawa, Hu Ruiling, Guo Zhongshang, Wei Xiaodan, Liu Zhifeng, Ji Xiaohui, Dias Alberto C P, Zhang Xiaoying
China and Portugal Joint Research Center, Shaanxi International Cooperation Demonstration Base, Shaanxi University of Technology, Hanzhong, Shaanxi 723000, China.
Shaanxi Key Laboratory of Catalysis, School of Chemical and Environment Science, Shaanxi University of Technology, Hanzhong, Shaanxi 723000, China.
ACS Appl Mater Interfaces. 2025 Jul 9;17(27):38859-38873. doi: 10.1021/acsami.5c05798. Epub 2025 Jun 26.
Spherical heterojunction nanocomposite materials are utilized to treat wound infections caused by drug-resistant bacteria by generating reactive oxygen species (ROS) and multiple cations (multiple inorganic or organic ions with positive points). However, there is an ongoing debate on the relative contributions of ROS and multiple cations toward antibacterial activity. In this study, the CuFeO/Cu@PEI/Ag (ZPA) nanocomposites were synthesized for releasing abundantO,OH, Fe, Cu, Ag, and polyethylenimine (PEI), and studied the contribution of the released ions to the bacteriostatic activity against drug sensitive (ATCC25923) and drug-resistant (ATCC43360). The results revealed that the antibacterial activity is attributed in the following order: multiple cations >O>OH > single cation. The antibacterial mechanism of the material involved leakage of the cytoplasmic content by damaging the bacterial cell wall, and the alteration of the secondary structure of the cell wall by multiple cations bound to the bacterial cell wall via electrostatic attraction. By healing drug-resistant -induced wound infection and completely eliminating bacterial burden after 11 days, in addition, ZPA also effectively polarized M1 type macrophages to M2 type in vivo to promote wound healing. Thus, our findings elucidate that multiple cations occupy an important position on the antibacterial properties of composite nanomaterials. Moreover, The ZPA represent a promising strategy for addressing drug-resistant -induced wound infections.
球形异质结纳米复合材料通过产生活性氧（ROS）和多种阳离子（多种带正电荷的无机或有机离子）来治疗由耐药细菌引起的伤口感染。然而，关于ROS和多种阳离子对抗菌活性的相对贡献仍存在争议。在本研究中，合成了CuFeO/Cu@PEI/Ag（ZPA）纳米复合材料以释放大量的O、OH、Fe、Cu、Ag和聚乙烯亚胺（PEI），并研究了释放的离子对药敏（ATCC25923）和耐药（ATCC43360）细菌抑菌活性的贡献。结果表明，抗菌活性的归因顺序如下：多种阳离子>O>OH>单一阳离子。该材料的抗菌机制包括通过破坏细菌细胞壁导致细胞质内容物泄漏，以及多种阳离子通过静电吸引与细菌细胞壁结合从而改变细胞壁的二级结构。此外，通过治愈耐药性引起的伤口感染并在11天后完全消除细菌负荷，ZPA还能在体内有效地将M1型巨噬细胞极化为M2型以促进伤口愈合。因此，我们的研究结果阐明了多种阳离子在复合纳米材料抗菌性能中占据重要地位。此外，ZPA代表了一种应对耐药性引起的伤口感染的有前景的策略。
