Chrysin's protective effect on the expression of , , , and genes against diazinon toxin in male rats: An experimental study.

BACKGROUND

Environmental pollution due to pesticides is a major health problem worldwide that causes toxicity. Chrysin (C) is a flavonoid that reduces the adverse effects caused by diazinon (D).

OBJECTIVE

To investigate the protective effects of C on the expression of , testis-specific gene antigen 10 (), deleted azoospermia-like gene () and A-Kinase anchoring protein 4 () genes in the testicular tissue of rats receiving D.

MATERIALS AND METHODS

In this experimental study, 42 Wistar rats (7-8 wk, 180-250 gr) were selected. This study was conducted in 2021 at the Pasteur Institute of Iran, Amol Research Center, Amol, Iran. Injections were performed 5 days a week for 4 wk. Random grouping of rats: 1) control: without any injection. 2) Sham: injection of 10% tween 80 solution as D solvent. 3) D: injection at a concentration of 20 mg/kg/BW. 4 and 5) C with low dose (C10 mg/kg/BW) and high dose (C20 mg/kg/BW). 6 and 7) D with low dose of C (D20 mg/kg/BW/ C10 mg/kg/BW) and D with high dose: (D20 mg/kg/BW/ C20 mg/kg/BW). Finally, the testes were examined in terms of histology and gene expression.

RESULTS

Histological results showed that D improved spermatogenesis after C intervention by reducing germinal cells, diameter, and lumen area. SPSS results showed that D reduced the expression of (p 0.0001), (p = 0.01) and (p 0.0001) genes, but increased the expression of gene (p 0.0001).

CONCLUSION

D has a negative effect on the expression of meiotic genes in rat testicular tissue, while C greatly reduces the adverse effects caused by D.