Mojibi Fatemeh, Fattahi Esmail, Jorsaraei Seyed Gholam Ali, Kazemi Sohrab, Tabari Maryam Gholamitabar
Department of Biology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran.
Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
Int J Reprod Biomed. 2025 Jun 10;23(3):275-288. doi: 10.18502/ijrm.v23i3.18778. eCollection 2025 Mar.
Environmental pollution due to pesticides is a major health problem worldwide that causes toxicity. Chrysin (C) is a flavonoid that reduces the adverse effects caused by diazinon (D).
To investigate the protective effects of C on the expression of , testis-specific gene antigen 10 (), deleted azoospermia-like gene () and A-Kinase anchoring protein 4 () genes in the testicular tissue of rats receiving D.
In this experimental study, 42 Wistar rats (7-8 wk, 180-250 gr) were selected. This study was conducted in 2021 at the Pasteur Institute of Iran, Amol Research Center, Amol, Iran. Injections were performed 5 days a week for 4 wk. Random grouping of rats: 1) control: without any injection. 2) Sham: injection of 10% tween 80 solution as D solvent. 3) D: injection at a concentration of 20 mg/kg/BW. 4 and 5) C with low dose (C10 mg/kg/BW) and high dose (C20 mg/kg/BW). 6 and 7) D with low dose of C (D20 mg/kg/BW/ C10 mg/kg/BW) and D with high dose: (D20 mg/kg/BW/ C20 mg/kg/BW). Finally, the testes were examined in terms of histology and gene expression.
Histological results showed that D improved spermatogenesis after C intervention by reducing germinal cells, diameter, and lumen area. SPSS results showed that D reduced the expression of (p 0.0001), (p = 0.01) and (p 0.0001) genes, but increased the expression of gene (p 0.0001).
D has a negative effect on the expression of meiotic genes in rat testicular tissue, while C greatly reduces the adverse effects caused by D.
农药造成的环境污染是全球范围内一个导致毒性的主要健康问题。白杨素（C）是一种黄酮类化合物，可减轻二嗪农（D）引起的不良反应。
研究C对接受D的大鼠睾丸组织中睾丸特异性基因抗原10（）、缺失无精子症样基因（）和A激酶锚定蛋白4（）基因表达的保护作用。
在本实验研究中，选取42只Wistar大鼠（7 - 8周龄，180 - 250克）。本研究于2021年在伊朗阿莫勒研究中心的伊朗巴斯德研究所进行。每周注射5天，持续4周。大鼠随机分组：1）对照组：不进行任何注射。2）假手术组：注射10%吐温80溶液作为D的溶剂。3）D组：以20毫克/千克体重的浓度注射。4和5）低剂量C组（C10毫克/千克体重）和高剂量C组（C20毫克/千克体重）。6和7）低剂量C的D组（D20毫克/千克体重/C10毫克/千克体重）和高剂量C的D组：（D20毫克/千克体重/C20毫克/千克体重）。最后，从组织学和基因表达方面对睾丸进行检查。
组织学结果显示，D通过减少生殖细胞、直径和管腔面积，在C干预后改善了精子发生。SPSS结果显示，D降低了（p < 0.0001）、（p = 0.01）和（p < 0.0001）基因的表达，但增加了基因的表达（p < 0.0001）。
D对大鼠睾丸组织中减数分裂基因的表达有负面影响，而C可大大减轻D引起的不良反应。
