Fu Shan, Chen Xiuwei, Jiang Yanhong, Dai Shengyue, Zhang Haizhen, Feng Shuang, Zhou Aimin
College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin, 150030 China.
Large-Scale Instrument and Equipment Sharing Service Platform, Northeast Agricultural University, Harbin, 150030 China.
Physiol Mol Biol Plants. 2025 May;31(5):823-833. doi: 10.1007/s12298-025-01605-2. Epub 2025 Jun 3.
Soil salinity is one of the main environmental limiting factors for plant growth and production. Sodium salt (NaCl) is the main salt in saline soils. The high-affinity K transporter (HKT1) is a Na-preferential transporter identified in multiple glycophyte plants, and it participates in salt tolerance through shoot Na extrusion. However, there has been limited research on the identification and characterization of HKT1 from halophytes. In this study, SlHKT1.1 was cloned and functionally characterized from the halophytic shrub . Sequence analysis revealed that SlHKT1.1 is a member of HKT1. Transient expression of SlHKT1.1 in tobacco leaves showed that it as a plasma membrane protein. Under NaCl and KCl stress, overexpression severely inhibited the root growth of transgenic poplar seedlings. Ion content measurements revealed significantly higher Na content in the roots of transgenic poplar seedlings than in wild-type roots. Correlation analysis showed that significant root growth inhibition was associated with higher Na accumulation in roots in transgenic poplar seedlings. Analyses of K content and flux rate revealed that SlHKT1.1 was not directly involved in the transport and accumulation of K. These studies suggest that SlHKT1.1, a plasma membrane Na-preferential transporter from , can be used for enhancing plant salt tolerance via tissue- or cell-specific expression.
The online version contains supplementary material available at 10.1007/s12298-025-01605-2.
土壤盐渍化是植物生长和生产的主要环境限制因素之一。钠盐（NaCl）是盐渍土壤中的主要盐分。高亲和性钾转运体（HKT1）是在多种甜土植物中鉴定出的一种优先转运钠的转运体，它通过地上部排钠参与耐盐性。然而，关于从盐生植物中鉴定和表征HKT1的研究有限。在本研究中，从盐生灌木中克隆了SlHKT1.1并对其进行了功能表征。序列分析表明SlHKT1.1是HKT1家族的一员。SlHKT1.1在烟草叶片中的瞬时表达表明它是一种质膜蛋白。在NaCl和KCl胁迫下，过表达严重抑制了转基因杨树幼苗的根系生长。离子含量测定显示，转基因杨树幼苗根系中的Na含量显著高于野生型根系。相关性分析表明，转基因杨树幼苗根系生长受到显著抑制与根系中较高的Na积累有关。对K含量和通量率的分析表明，SlHKT1.1不直接参与K的运输和积累。这些研究表明，SlHKT1.1是一种来自盐生植物的质膜优先转运钠的转运体，可通过组织或细胞特异性表达用于提高植物的耐盐性。
在线版本包含可在10.1007/s12298-025-01605-2获取的补充材料。
