Shamsudduha Md, Rahman Md Mahfuzur, Naher Jannatun, Barkat Azizul Islam, Akter Sumaiya, Alam Mohammad Shamimul
Department of Zoology (Genetics and Molecular Biology Branch), University of Dhaka, Dhaka, Bangladesh.
J Adv Vet Anim Res. 2025 Mar 26;12(1):280-286. doi: 10.5455/javar.2025.l894. eCollection 2025 Mar.
The present study was designed to conduct molecular and morphological identification of cat fleas () from Bangladesh along with nucleotide polymorphism and phylogenetic analysis.
Samples were collected from two hosts (cat and human). The species was identified through morphological studies first, and then DNA was extracted for subsequent molecular analysis. A part of the mitochondrial gene was amplified by polymerase chain reaction using extracted DNA as a template. The amplified region was sequenced using the Sanger dideoxy method. The sequence was subjected to NCBI BLASTn search. BioEdit and MEGA 11 software were used for multiple sequence alignment (MSA) and generating a phylogenetic tree.
Morphological features such as shape, size, and appendages showed similarity with The metatibial formula of chaetotaxy (2-2-2-2-1-3) was confirmed for species-level identification. NCBI BLASTn search showed the highest sequence identity with the available sequence of . such as 99.78% (NC_049858.1) and 99.12% (MW420044.1 and MK941844.1) with 100% query coverage. MSA of . sequences from different geographical distributions show their sequence affinities with each other, and the phylogenetic tree presents their relationship with each other.
Both morphological and molecular studies clearly indicate the identity and confirmation of cat flea (. from Bangladesh.
本研究旨在对来自孟加拉国的猫蚤（）进行分子和形态学鉴定，并进行核苷酸多态性和系统发育分析。
样本从两种宿主（猫和人）中采集。首先通过形态学研究鉴定物种，然后提取DNA用于后续分子分析。以提取的DNA为模板，通过聚合酶链反应扩增线粒体基因的一部分。使用桑格双脱氧法对扩增区域进行测序。将该序列进行NCBI BLASTn搜索。使用BioEdit和MEGA 11软件进行多序列比对（MSA）并生成系统发育树。
形态特征如形状、大小和附肢与显示出相似性。确认了用于物种水平鉴定的后胫鬃序公式（2 - 2 - 2 - 2 - 1 - 3）。NCBI BLASTn搜索显示与的可用序列具有最高的序列同一性。例如与99.78%（NC_049858.1）、99.12%（MW420044.1和MK941844.1）具有100%的查询覆盖率。来自不同地理分布的序列的MSA显示它们彼此之间的序列亲和力，并且系统发育树呈现了它们彼此之间的关系。
形态学和分子研究均清楚地表明了来自孟加拉国的猫蚤（）的身份并予以确认。
