Shinoda J, Miwa Y, Sakai N, Yamada H, Shima H, Kato K, Takahashi M, Shimokawa K
J Neurosurg. 1985 Nov;63(5):733-9. doi: 10.3171/jns.1985.63.5.0733.
Indirect immunoperoxidase staining by the peroxidase-antiperoxidase (PAP) technique was carried out on 23 human primary intracranial germ-cell tumors (17 germinomas, one embryonal carcinoma, one yolk-sac tumor, three teratomas, and one teratoma with embryonal carcinoma) and on six human primary pineal non-germ-cell tumors (one pineocytoma, two pineoblastomas, two astrocytomas, and one glioblastoma multiforme). The technique used specific rabbit antisera against placental alkaline phosphatase (PLAP), alpha-fetoprotein (AFP), and human chorionic gonadotropin (HCG). Thirteen of 17 intracranial germinomas (76.5%) showed positive staining for PLAP mainly on the tumor cell membrane. In six primary intracranial non-seminomatous germ-cell tumors, there was weak positive staining indicating the presence of PLAP in only a few cells of one embryonal carcinoma, and in some glandular epithelial cells of one teratoma; this staining was limited to the cytoplasm. None of the other six primary pineal non-germ-cell tumors showed any positive PLAP reaction. From these results, PLAP was shown to be very useful in histopathology as a diagnostic tumor marker of intracranial germinoma. Positive AFP staining was seen in several yolk-sac tumor cells and a few embryonal carcinoma cells. However, no intracranial germinomas and non-germ-cell tumors of the pineal region showed positive reaction. As for HCG, only one suprasellar germinoma and one pineal embryonal carcinoma among 29 specimens contained a few positive-staining tumor cells.
采用过氧化物酶抗过氧化物酶（PAP）技术对23例人类原发性颅内生殖细胞肿瘤（17例生殖细胞瘤、1例胚胎癌、1例卵黄囊瘤、3例畸胎瘤以及1例含胚胎癌成分的畸胎瘤）和6例人类原发性松果体非生殖细胞肿瘤（1例松果细胞瘤、2例松果体母细胞瘤、2例星形细胞瘤以及1例多形性胶质母细胞瘤）进行间接免疫过氧化物酶染色。该技术使用了针对胎盘碱性磷酸酶（PLAP）、甲胎蛋白（AFP）和人绒毛膜促性腺激素（HCG）的特异性兔抗血清。17例颅内生殖细胞瘤中有13例（76.5%）主要在肿瘤细胞膜上显示PLAP阳性染色。在6例原发性颅内非精原细胞生殖细胞肿瘤中，仅1例胚胎癌的少数细胞以及1例畸胎瘤的一些腺上皮细胞呈弱阳性染色，提示存在PLAP；这种染色局限于细胞质。其他6例原发性松果体非生殖细胞肿瘤均未显示任何PLAP阳性反应。从这些结果来看，PLAP在组织病理学中被证明是一种非常有用的颅内生殖细胞瘤诊断肿瘤标志物。在几个卵黄囊瘤细胞和少数胚胎癌细胞中可见AFP阳性染色。然而，松果体区的颅内生殖细胞瘤和非生殖细胞肿瘤均未显示阳性反应。至于HCG，在29个标本中，只有1例鞍上生殖细胞瘤和1例松果体胚胎癌含有少数阳性染色的肿瘤细胞。
