Zhang Huan, Dai Zhuoru, Zhang Xiaochen, Shang Meiqi, Gao Xiaoru, Ma Ruiqi, Zhao Limeng, Zhang Xiaoli, Liu Qingchang, Zhai Hong, Gao Shaopei, Zhao Ning, Cao Qinghe, Li Qiang, He Shaozhen
Key Laboratory of Sweet Potato Biology and Biotechnology, Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education, College of Agronomy & Biotechnology, China Agricultural University, Beijing, 10093, China.
Sanya Institute of China Agricultural University, Sanya, 572025, China.
Adv Sci (Weinh). 2025 Sep;12(33):e15202. doi: 10.1002/advs.202415202. Epub 2025 Jun 26.
Sweet potato (Ipomoea batatas) is a globally important autohexaploid root and tuber crop. Fusarium root rot threatens its entire growth, harvest, and storage period, thereby reducing yield and quality. Therefore, a deeper understanding of Fusarium pathogenicity and sweet potato defense is urgently required. Here, two single nucleotide polymorphisms are identified within the promoter region of the I. batatas CHY zinc-finger and ring protein1 (IbCHYR1) gene that encode an E3 ubiquitin ligase linked to root rot resistance. In susceptible varieties, the high dosage allele Pro::IbCHYR1 leads to increased expression of IbCHYR1. Overexpression of IbCHYR1 increases susceptibility to root rot and Fusarium wilt. IbCHYR1 interacts with the I. batatas CCCH-type zinc-finger protein (IbZnFR) to promote its degradation. IbZnFR confers resistance to root rot and Fusarium wilt and improves yield by more than 10%. The high dosage Pro::IbZnFR allele is associated with resistance to root rot disease. Moreover, FfRlpA2, a conserved Fusarium effector, is identified as a protease inhibitor that stabilizes and hijacks IbCHYR1 to degrade IbZnFR, thereby inhibiting multiple defense pathways. These findings provide insights into Fusarium pathogenicity and a genetic basis for root rot research and improvement of disease-resistant sweet potato varieties.
甘薯（Ipomoea batatas）是一种全球重要的同源六倍体块根和块茎作物。镰刀菌根腐病威胁着其整个生长、收获和储存期，从而降低产量和品质。因此，迫切需要更深入地了解镰刀菌的致病性和甘薯的防御机制。在这里，在甘薯CHY锌指和环蛋白1（IbCHYR1）基因的启动子区域内鉴定出两个单核苷酸多态性，该基因编码一种与根腐病抗性相关的E3泛素连接酶。在易感品种中，高剂量等位基因Pro::IbCHYR1导致IbCHYR1表达增加。IbCHYR1的过表达增加了对根腐病和枯萎病的易感性。IbCHYR1与甘薯CCCH型锌指蛋白（IbZnFR）相互作用以促进其降解。IbZnFR赋予对根腐病和枯萎病的抗性，并使产量提高10%以上。高剂量的Pro::IbZnFR等位基因与根腐病抗性相关。此外，FfRlpA2是一种保守的镰刀菌效应子，被鉴定为一种蛋白酶抑制剂，它稳定并劫持IbCHYR1以降解IbZnFR，从而抑制多种防御途径。这些发现为镰刀菌的致病性提供了见解，并为根腐病研究和抗病甘薯品种的改良提供了遗传基础。
