Kakikawa Makiko, Kenmochi Ami, Yamada Sotoshi
Institute of Science and Engineering, Kanazawa University, Kanazawa, Japan.
Graduate school of Natural Science and Technology, Kanazawa University, Kanazawa, Japan.
Electromagn Biol Med. 2025 Jun 26:1-9. doi: 10.1080/15368378.2025.2523773.
Previous studies indicated that magnetic fields (MF) could alter the conformation of membrane proteins such as some drug influx transporters and affect their function. In this study, we focused on two mutants of receptor gene related to feeding behavior in ( to investigate the effect of 60 Hz, 50 mT MF on a receptor in neurotransmission. The wild-type laboratory strain Bristol N2 in forages solitary on the lawn, whereas mutant of N2 aggregate on food and take on social feeding behavior because of its altered function. Under MF exposure, the number of aggregates exhibiting social feeding behavior on two mutants, IM222 and DA609, was decreased by 40% and 25%, respectively. Meanwhile, is not involved in the behavior to attractant diacetyl in , and the moving speed and reach rate to attractant in two mutants was not affected by MF exposure for up to 90 min. The results indicate that MF can affect the altered function of NPR-1 in mutant strains and partially change the feeding behaviour of the mutants from social to solitary. Furthermore, considering the position of these amino acid substitutions in NPR-1 mutant receptors, the extracellular parts of membrane protein may be more affected by MF than the transmembrane part.
先前的研究表明，磁场（MF）可以改变膜蛋白的构象，如一些药物流入转运体，并影响其功能。在本研究中，我们聚焦于秀丽隐杆线虫（Caenorhabditis elegans）中与进食行为相关的受体基因的两个突变体，以研究60 Hz、50 mT的磁场对神经传递中一种受体的影响。秀丽隐杆线虫的野生型实验室菌株布里斯托尔N2在大肠杆菌草坪上单独觅食，而N2的一个突变体由于其功能改变，会聚集在食物上并表现出群居进食行为。在磁场暴露下，IM222和DA609这两个突变体上表现出群居进食行为的聚集体数量分别减少了40%和25%。同时，秀丽隐杆线虫对引诱剂二乙酰的行为不涉及该受体，并且在长达90分钟的磁场暴露下，两个突变体对引诱剂的移动速度和到达率不受影响。结果表明，磁场可以影响突变菌株中NPR-1的改变功能，并部分改变秀丽隐杆线虫突变体的进食行为，使其从群居变为单独进食。此外，考虑到这些氨基酸取代在NPR-1突变受体中的位置，膜蛋白的细胞外部分可能比跨膜部分更容易受到磁场的影响。
