Zhang Yuelong, Ren Xunshan, Zhuang Huangming, Li Huajie, Adam Miradj Siddick, Feng Rongling, Zhu Junming, Zhou Panghu
Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan, China.
PLoS One. 2025 Jun 26;20(6):e0325704. doi: 10.1371/journal.pone.0325704. eCollection 2025.
Osteoarthritis (OA), characterized by synovial inflammation, articular cartilage degeneration, and structural changes of subchondral bone and periarticular tissues, represents a major unmet clinical challenge. Targeting senescent chondrocytes has emerged as a promising therapeutic strategy of OA. Human umbilgratical cord mesenchymal stem cells (hUCMSCs) have shown potential in OA treatment through paracrine mechanisms, but their clinical translation is limited by challenges in cell viability control and safety concerns. hUCMSCs decellular extracellular matrix (hUCMSCs-dECM) can target senescent chondrocytes to alleviate senescence in OA. Stimulator of interferon gene (STING) can promote chondrocyte senescence in OA through the activation of NF-κB signaling, and inhibition of STING may provide a novel approach for OA treatment. Here, we demonstrated that hUCMSCs-dECM attenuated chondrocyte senescence in vivo and in vitro by inhibiting the STING-NF-κB pathway, which would provide a novel approach for OA treatment.
骨关节炎（OA）以滑膜炎症、关节软骨退变以及软骨下骨和关节周围组织的结构改变为特征，是一个尚未得到充分解决的重大临床挑战。靶向衰老软骨细胞已成为一种有前景的OA治疗策略。人脐带间充质干细胞（hUCMSCs）已通过旁分泌机制在OA治疗中显示出潜力，但其临床转化受到细胞活力控制方面的挑战和安全性问题的限制。hUCMSCs去细胞外基质（hUCMSCs-dECM）可靶向衰老软骨细胞以减轻OA中的衰老。干扰素基因刺激物（STING）可通过激活NF-κB信号通路促进OA中的软骨细胞衰老，抑制STING可能为OA治疗提供一种新方法。在此，我们证明hUCMSCs-dECM通过抑制STING-NF-κB途径在体内和体外减轻软骨细胞衰老，这将为OA治疗提供一种新方法。
