Kriangsaksri Ruttanaporn, Taengchaiyaphum Suparat, Payomhom Pattaree, Thaiue Dararat, Itsathitphaisarn Ornchuma, Sritunyalucksana Kallaya, Katewongsa Kanlaya Prapainop
Department of Biochemistry, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand.
Aquatic Animal Health Research Team (AQHT), Integrative Aquaculture Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Yothi Office, Rama VI Road, Ratchathewi, Bangkok 10400, Thailand.
ACS Biomater Sci Eng. 2025 Jul 14;11(7):4279-4292. doi: 10.1021/acsbiomaterials.5c00928. Epub 2025 Jun 26.
White spot syndrome virus (WSSV) is one of the most devastating pathogens affecting shrimp. Within a short time, it leads to a hundred percent mortality rate, which causes substantial economic losses. PmRab7 has been reported to bind to the envelope protein of WSSV, VP28, resulting in a reduction of viral replication. In order to apply PmRab7 in shrimp feed, the development of delivery systems is crucial. Poly(lactic--glycolic acid) (PLGA) is a biodegradable polymer extensively studied for drug delivery in the form of nanoparticles or microspheres (MSs). Despite its potential, PLGA has not been previously reported for antiviral use in shrimp. This study is the first to demonstrate the potential use of PLGA and chitosan-coated PLGA (PLGA/CS) MSs for the delivery of PmRab7 in shrimp. Both PLGA and PLGA/CS were optimized and characterized to allow for a sustained release of encapsulated PmRab7. Initial in vitro and in vivo evaluations demonstrated that both MSs are safe for use in shrimp, can sustain the release of PmRab7, and enhance its antiviral activity as shown by a decrease in the mortality rate in shrimp. The development of these MSs has the potential to significantly enhance disease control in shrimp aquaculture, leading to more effective and sustainable practices that will ultimately bolster the industry's growth and long-term stability.
白斑综合征病毒（WSSV）是影响对虾的最具毁灭性的病原体之一。在短时间内，它会导致100%的死亡率，造成巨大的经济损失。据报道，凡纳滨对虾Rab7（PmRab7）与WSSV的包膜蛋白VP28结合，从而减少病毒复制。为了将PmRab7应用于对虾饲料中，递送系统的开发至关重要。聚乳酸-乙醇酸共聚物（PLGA）是一种可生物降解的聚合物，已被广泛研究用于以纳米颗粒或微球（MSs）形式的药物递送。尽管有其潜力，但PLGA此前尚未报道用于对虾的抗病毒应用。本研究首次证明了PLGA和壳聚糖包被的PLGA（PLGA/CS）微球在对虾中递送PmRab7的潜在用途。对PLGA和PLGA/CS都进行了优化和表征，以实现包封的PmRab7的持续释放。初步的体外和体内评估表明，两种微球用于对虾都是安全的，能够持续释放PmRab7，并增强其抗病毒活性，表现为对虾死亡率降低。这些微球的开发有可能显著加强对虾养殖中的疾病控制，带来更有效和可持续的做法，最终促进该行业的增长和长期稳定。
