Seferli Marina, Lefkaki Melina, Manousakis Vasileios, Ofrydopoulou Anna, Shiels Katie, Saha Sushanta Kumar, Krey Grigorios, Kamidis Nikolaos, Stamatis Nikolaos, Anastasiadou Chryssa, Tsoupras Alexandros
Hephaestus Laboratory, Department of Chemistry, International Hellenic University, Kavala University Campus, 65404 Kavala, Greece.
Centre for Applied Bioscience Research, Technological University of the Shannon-Midlands Midwest, Moylish Park, V94 E8YF Limerick, Ireland.
Pharmaceuticals (Basel). 2025 Jun 2;18(6):835. doi: 10.3390/ph18060835.
Chronic diseases linked to inflammation, such as cardiovascular disease (CVD) and cancer, continue to pose major public health challenges due to their high mortality rates. There is growing interest in natural bioactive compounds, particularly those derived from plants, as potential therapeutic or preventive agents due to their low toxicity profiles. This study aimed to explore two freshwater plants- (Indian lotus) and sp.-as potential sources of bioactive compounds with antioxidant, anti-inflammatory, and antithrombotic properties. While has established but incompletely characterized biofunctional properties, sp. remains largely unexplored in this context. Amphiphilic extracts from both plant species were analyzed for phenolic and lipid constituents, including unsaturated fatty acids, polar lipids, and carotenoids. Antioxidant capacity was evaluated using DPPH, ABTS, and FRAP assays. Anti-inflammatory and antithrombotic activities were assessed via platelet aggregation assays using PAF and ADP agonists. Structural characterization was performed using (FT-IR) and liquid chromatography-mass spectroscopy (LC-MS) to support structure-activity relationship (SAR) analysis. Extracts, particularly from sp., showed potent antiplatelet activity against PAF and ADP. LC-MS revealed the presence of polar lipids rich in monounsaturated and omega-3 polyunsaturated fatty acids, with a favorable omega-6/omega-3 ratio, especially in sp., correlating with strong anti-inflammatory potential. High levels of total phenolics and carotenoids were observed, aligning with substantial antioxidant capacity in both species. These findings suggest that and sp. are promising sources of bioactive compounds with potential applications in functional foods, cosmetics, and pharmaceuticals targeting inflammation- and thrombosis-related chronic diseases. Further studies are warranted to confirm their safety and efficacy.
与炎症相关的慢性疾病，如心血管疾病（CVD）和癌症，因其高死亡率继续构成重大的公共卫生挑战。天然生物活性化合物，特别是那些源自植物的化合物，由于其低毒性，作为潜在的治疗或预防剂越来越受到关注。本研究旨在探索两种淡水植物——（印度莲花）和——作为具有抗氧化、抗炎和抗血栓特性的生物活性化合物的潜在来源。虽然已经确定了的生物功能特性，但尚未完全表征，而在这方面基本上仍未被探索。对这两种植物的两亲性提取物进行了酚类和脂质成分分析，包括不饱和脂肪酸、极性脂质和类胡萝卜素。使用DPPH、ABTS和FRAP测定法评估抗氧化能力。通过使用PAF和ADP激动剂的血小板聚集试验评估抗炎和抗血栓活性。使用（傅里叶变换红外光谱）和液相色谱 - 质谱联用（LC - MS）进行结构表征，以支持构效关系（SAR）分析。提取物，特别是来自的提取物，对PAF和ADP显示出强大的抗血小板活性。LC - MS显示存在富含单不饱和和ω - 3多不饱和脂肪酸的极性脂质，具有良好的ω - 6/ω - 3比率，特别是在中，这与强大的抗炎潜力相关。观察到高水平的总酚类和类胡萝卜素，这与两种植物的大量抗氧化能力一致。这些发现表明，和是具有生物活性化合物的有前途的来源，在针对炎症和血栓相关慢性疾病的功能性食品、化妆品和药物中具有潜在应用。有必要进一步研究以确认它们的安全性和有效性。
