Dahlén G, Hofstad T
Scand J Dent Res. 1977 May;85(4):272-8. doi: 10.1111/j.1600-0722.1977.tb00563.x.
Lipopolysaccharides (LPS) prepared from a strain of Bacteroides oralis, a strain of Fusobacterium necrophorum, and a strain of F. nucleatum, all isolated from an infected root canal in monkey (Macaca cynomolgus), were examined for endotoxic activities using primary skin reactions in rabbits and induction of leukocyte chemotaxis in rats. LPS of B. oralis showed considerably lower ability to cause skin inflammation than LPS of the fusobacteria. However, the leukotactic effect of the LPS preparations as determined by the wound chamber method in rats was approximately of the same proportion. In both tests the reactions were compared with those of commercial LPS of Salmonella typhi. This study shows that endotoxic LPS can be isolated from oral Gram-negative bacteria, which have infected the root canal. Therefore LPS may play a role in the development and maintenance of chronic inflammation of the periapical tissues.
从猕猴（食蟹猴）感染根管中分离出的一株口腔拟杆菌、一株坏死梭杆菌和一株具核梭杆菌制备的脂多糖（LPS），采用兔原发性皮肤反应和大鼠白细胞趋化性诱导法检测其内毒素活性。口腔拟杆菌的LPS引起皮肤炎症的能力明显低于梭杆菌的LPS。然而，通过大鼠伤口小室法测定，LPS制剂的白细胞趋化作用比例大致相同。在两项试验中，均将反应与伤寒沙门氏菌的商用LPS反应进行比较。本研究表明，可从感染根管的口腔革兰氏阴性菌中分离出内毒素LPS。因此，LPS可能在根尖周组织慢性炎症的发生和维持中起作用。
