长链非编码RNA ST6GALNAC3通过chi-miR-24-3p/ID4轴抑制绒山羊毛囊中真皮成纤维细胞的增殖和迁移。

LncRNA ST6GALNAC3 inhibits dermal fibroblast proliferation and migration in cashmere goat hair follicles via chi-miR-24-3p/ID4 axis.

作者信息

Ma Rong, Ma Qing, Zhang Lu, Ma Bingjie, Bao Xuxu, Zhang Yiming, Wang Le, Lv Qi, Wang Zhiying, Wang Ruijun, Su Rui, Zhao Yanhong, Shang Fangzheng, Wang Yu, Zhang Yanjun

机构信息

College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.

Science and Technology Development Center of Ulanqab, Ulanqab, China.

出版信息

Anim Biosci. 2025 Jun 24. doi: 10.5713/ab.25.0115.

DOI:10.5713/ab.25.0115

PMID:40575977

Abstract

OBJECTIVE

Dermal papilla is formed by the continuous proliferation and differentiation of dermal fibroblasts, which is the key to the normal development of hair follicles. Therefore, this study aims to elucidate the role of lncRNA ST6GALNAC3, which is significantly differentially expressed during the secondary hair follicle development stage in cashmere goats, on dermal fibroblasts, and to thoroughly analyze the regulatory mechanism of this lncRNA.

METHODS

Taking into account the characteristics of secondary hair follicle development, we conducted a screen for lncRNAs that are associated with this process. CCK8, EdU, and flow cytometry detected the effects of lncRNA ST6GALNAC3 on cell proliferation and migration. Subsequently, we employed bioinformatics analysis to predict the target miRNAs of lncRNA ST6GALNAC3 and the target genes of these miRNAs, respectively, and initially constructed the regulatory axis of lncRNA ST6GALNAC3-chi-miR-24-3p-ID4. Subsequently, luciferase reporter assays and rescue experiments were performed to confirm the regulatory axis at both molecular and cellular levels, thus elucidating the mechanism by which lncRNA ST6GALNAC3 regulates dermal fibroblasts.

RESULTS

A total of 158 lncRNAs related to secondary hair follicle morphogenesis were identified. Among them, lncRNA ST6GALNAC3 was significantly differentially expressed on embryonic day 75 and significantly inhibited the proliferation and migration of dermal fibroblasts. The results showed that lncRNA ST6GALNAC3 could target chi-miR-24-3p, and chi-miR-24-3p could target the ID4 gene. Subsequently, the results of luciferase reporter assay and rescue assay showed that chi-miR-24-3p had binding sites with both lncRNA ST6GALNAC3 and ID4, and lncRNA ST6GALNAC3 could indirectly regulate the proliferation and migration of dermal fibroblasts through chi-miR-24-3p/ID4 axis.

CONCLUSION

LncRNA ST6GALNAC3 inhibits the proliferation and migration of dermal fibroblasts through the chi-miR-24-3p/ID4 axis, thus suppressing the formation of dermal papilla structures and influencing the morphogenesis of secondary hair follicles during the embryonic period.

摘要

目的

真皮乳头由真皮成纤维细胞持续增殖分化形成，是毛囊正常发育的关键。因此，本研究旨在阐明在绒山羊次级毛囊发育阶段显著差异表达的lncRNA ST6GALNAC3对真皮成纤维细胞的作用，并深入分析该lncRNA的调控机制。

方法

考虑到次级毛囊发育的特点，我们对与该过程相关的lncRNAs进行了筛选。CCK8、EdU和流式细胞术检测lncRNA ST6GALNAC3对细胞增殖和迁移的影响。随后，我们分别采用生物信息学分析预测lncRNA ST6GALNAC3的靶标miRNAs以及这些miRNAs的靶标基因，并初步构建了lncRNA ST6GALNAC3-chi-miR-24-3p-ID4调控轴。随后，进行荧光素酶报告基因检测和拯救实验，在分子和细胞水平上确认该调控轴，从而阐明lncRNA ST6GALNAC3调控真皮成纤维细胞的机制。

结果

共鉴定出158个与次级毛囊形态发生相关的lncRNAs。其中，lncRNA ST6GALNAC3在胚胎第75天显著差异表达，并显著抑制真皮成纤维细胞的增殖和迁移。结果表明，lncRNA ST6GALNAC3可以靶向chi-miR-24-3p，而chi-miR-24-3p可以靶向ID4基因。随后，荧光素酶报告基因检测和拯救实验结果表明，chi-miR-24-3p与lncRNA ST6GALNAC3和ID4均具有结合位点，lncRNA ST6GALNAC3可以通过chi-miR-24-3p/ID4轴间接调控真皮成纤维细胞的增殖和迁移。