Mirzaei Fatemeh, Jalili Cyrus, Khodadadi Iraj, Hosseini Nashmin Fayazi, Majdoub Nesrine, Naseri Nima, Mirzaei Amir, Abbasi Ebrahim
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Biol Trace Elem Res. 2025 Jun 27. doi: 10.1007/s12011-024-04398-9.
This experiment was carried out to investigate the effects of ZnO nanoparticles (ZnO NPs) on metabolic parameters, oxidative stress, apoptosis, and pathophysiological alterations of the pancreas in diabetic rats. Nanoparticle was synthesized and its characterizations were determined. We evaluate the toxicity and useful dosage of the ZnO NPs in Wistar male rats. Our experiment showed that 5 mg/kg had a useful effect and was not toxic. Hence, in the next step, the Wistar male rats were randomly divided into 3 groups as follows: (1) control rats (C); (2) diabetic rats (D), (3) diabetic rats received 5 mg/kg NP. After 4 weeks, animals were sacrificed, and blood chemical factors were measured. The oxidative stress, inflammatory, and apoptosis pathways were evaluated. Insulin and glucagon-like peptide-1 (GLP-1) mass was evaluated by immunofluorescence. The morphological changes were evaluated using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, hematoxylin and eosin (H&E), and aldehyde fuchsin. ZnO NP acts as an insulin sensitizer and normalizes blood glucose, GLP-1 levels as well as apoptosis, oxidative stress, and inflammatory pathway gene expression in diabetic rats. ZnO NP also alleviates the pathological alterations in the pancreas. This study showed that a low dose of ZnO NPs protects pancreatic β cells from oxidative stress and apoptosis. Administration of ZnO NP also normalized the pathophysiological alteration of the pancreas, thus normalizing metabolic abnormality.
本实验旨在研究氧化锌纳米颗粒（ZnO NPs）对糖尿病大鼠代谢参数、氧化应激、细胞凋亡及胰腺病理生理改变的影响。合成了纳米颗粒并对其进行了表征测定。我们评估了ZnO NPs在雄性Wistar大鼠中的毒性及有效剂量。我们的实验表明，5 mg/kg具有有益作用且无毒。因此，在下一步实验中，将雄性Wistar大鼠随机分为3组如下：（1）对照大鼠（C）；（2）糖尿病大鼠（D），（3）接受5 mg/kg纳米颗粒的糖尿病大鼠。4周后，处死动物并测量血液化学因子。评估氧化应激、炎症和细胞凋亡途径。通过免疫荧光评估胰岛素和胰高血糖素样肽-1（GLP-1）的含量。使用末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）法、苏木精和伊红（H&E）染色以及醛复红对形态学变化进行评估。ZnO NP可作为胰岛素增敏剂，使糖尿病大鼠的血糖、GLP-1水平以及细胞凋亡、氧化应激和炎症途径基因表达恢复正常。ZnO NP还可减轻胰腺的病理改变。本研究表明，低剂量的ZnO NPs可保护胰腺β细胞免受氧化应激和细胞凋亡的影响。给予ZnO NP还可使胰腺的病理生理改变恢复正常，从而使代谢异常恢复正常。
