Rayyif Sajjad Mohsin I, Mohammed Hamzah Basil, Curuțiu Carmen, Bîrcă Alexandra Cătălina, Grumezescu Alexandru Mihai, Vasile Bogdan Ștefan, Dițu Lia Mara, Lazăr Veronica, Chifiriuc Mariana Carmen, Mihăescu Grigore, Holban Alina Maria
Microbiology & Immunology Department, Faculty of Biology, University of Bucharest, 77206 Bucharest, Romania.
Research Institute of the University of Bucharest-ICUB, University of Bucharest, 050657 Bucharest, Romania.
Materials (Basel). 2021 Jun 4;14(11):3084. doi: 10.3390/ma14113084.
Zinc oxide (ZnO) nanoparticles (NPs) have been investigated for various skin therapies in recent years. These NPs can improve the healing and modulate inflammation in the wounds, but the mechanisms involved in such changes are yet to be known. In this study, we have designed a facile ZnO nano-coated dressing with improved antimicrobial efficiency against typical wound pathogens involved in biofilm and chronic infections. ZnO NPs were obtained by hydrothermal method and characterized by X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and Fourier-transform infrared spectroscopy. Antibacterial and antibiofilm effects were evaluated against laboratory and clinical isolates of significant Gram-negative ( and ) and Gram-positive ( and ) opportunistic pathogens, by quantitative methods. Our results have shown that the developed dressings have a high antibacterial efficiency after 6-24 h of contact when containing 0.6 and 0.9% ZnO NPs and this effect is similar against reference and clinical isolates. Moreover, biofilm development is significantly impaired for up to three days of contact, depending on the NPs load and microbial species. These results show that ZnO-coated dressings prevent biofilm development of main wound pathogens and represent efficient candidates for developing bioactive dressings to fight chronic wounds.
近年来，氧化锌（ZnO）纳米颗粒（NPs）已被用于多种皮肤治疗的研究。这些纳米颗粒可以促进伤口愈合并调节伤口炎症，但其中涉及的机制尚不清楚。在本研究中，我们设计了一种简便的ZnO纳米涂层敷料，它对参与生物膜和慢性感染的典型伤口病原体具有更高的抗菌效率。通过水热法制备了ZnO纳米颗粒，并通过X射线衍射、扫描电子显微镜、透射电子显微镜和傅里叶变换红外光谱对其进行了表征。采用定量方法评估了该敷料对重要革兰氏阴性（和）及革兰氏阳性（和）机会性病原体的实验室分离株和临床分离株的抗菌和抗生物膜效果。我们的结果表明，当含有0.6%和0.9%的ZnO纳米颗粒时，所开发的敷料在接触6 - 24小时后具有较高的抗菌效率，并且对参考菌株和临床分离株的效果相似。此外，根据纳米颗粒负载量和微生物种类的不同，在长达三天的接触时间内，生物膜的形成均受到显著抑制。这些结果表明，ZnO涂层敷料可防止主要伤口病原体形成生物膜，是开发用于治疗慢性伤口的生物活性敷料的有效候选材料。
