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在真菌中，光通过可变剪接控制基因功能。

Light controls gene functions through alternative splicing in fungi.

作者信息

Li Yifan, Lu Huanhong, Guo Degang, Li Xiaoyan, Qi Fei, Zhang Jian, Fischer Reinhard, Shen Qirong, Yu Zhenzhong

机构信息

Jiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Educational Ministry Engineering Center of Resource-saving fertilizers, Department of Plant Nutrition and Fertilizer Science, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China.

State Key Laboratory of Cellular Stress Biology, Department of Genetics and Developmental Biology, School of Life Sciences, Xiamen University, Xiamen 361102, China.

出版信息

Proc Natl Acad Sci U S A. 2025 Jul;122(26):e2500966122. doi: 10.1073/pnas.2500966122. Epub 2025 Jun 27.

DOI:10.1073/pnas.2500966122
PMID:40577119
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12232718/
Abstract

Light controls important biological processes in fungi by regulating transcriptional gene activation. Here, we found that beyond the regulation of mRNA transcript abundance, light regulates alternative splicing (AS) in the filamentous fungi , and . Blue light-regulated AS was involved in ergothioneine biosynthesis and conidiation in , which required the blue light receptor BLR1. Blue light activated the MAPK HOG (Sak) pathway which then transmitted the signal via the serine/threonine kinase SRK1 to the AS key regulator SRP1. SRK1 and SRP1 are important for light-induced conidiation. The light-activated HOG pathway led to an increase of the SRK1 protein level and its phosphorylation status. Phosphorylated SRK1 translocated from the cytoplasm to the nucleus to interact with SRP1, thereby regulating AS efficiency. This study unravels another level of complexity of fungal environmental sensing and responses and also first describes the entire cascade from an environmental signal to the splicing machinery.

摘要

光通过调节转录基因激活来控制真菌中的重要生物学过程。在这里，我们发现，除了调节mRNA转录本丰度外，光还调节丝状真菌中的可变剪接（AS）。蓝光调节的AS参与了麦角硫因的生物合成和分生孢子形成，这需要蓝光受体BLR1。蓝光激活了MAPK HOG（Sak）途径，然后该途径通过丝氨酸/苏氨酸激酶SRK1将信号传递给AS关键调节因子SRP1。SRK1和SRP1对光诱导的分生孢子形成很重要。光激活的HOG途径导致SRK1蛋白水平及其磷酸化状态增加。磷酸化的SRK1从细胞质转移到细胞核与SRP1相互作用，从而调节AS效率。这项研究揭示了真菌环境感知和反应的另一个复杂层面，并且首次描述了从环境信号到剪接机制的整个级联过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ef5/12232718/530c9fe19c45/pnas.2500966122fig05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ef5/12232718/5f748a1759b7/pnas.2500966122fig01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ef5/12232718/f041300e5100/pnas.2500966122fig02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ef5/12232718/6d054631d7e1/pnas.2500966122fig03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ef5/12232718/86dd9e44637d/pnas.2500966122fig04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ef5/12232718/530c9fe19c45/pnas.2500966122fig05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ef5/12232718/5f748a1759b7/pnas.2500966122fig01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ef5/12232718/f041300e5100/pnas.2500966122fig02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ef5/12232718/6d054631d7e1/pnas.2500966122fig03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ef5/12232718/86dd9e44637d/pnas.2500966122fig04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ef5/12232718/530c9fe19c45/pnas.2500966122fig05.jpg

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2
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mLife. 2023 Dec 4;2(4):365-377. doi: 10.1002/mlf2.12089. eCollection 2023 Dec.
3
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PLoS Genet. 2024 May 20;20(5):e1011282. doi: 10.1371/journal.pgen.1011282. eCollection 2024 May.
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