Pu Chunmei, Li Shiyang, Cao Xinlin, Chen Jun, Wang Peng, Wu Jiang
Precise Synthesis and Function Development Key Laboratory of Sichuan Province, College of Chemistry and Chemical Engineering, China West Normal University, Shida Road 1#, Nanchong 637009, PR. China.
Shijiazhuang Zangnuo Pharmaceutical Co., Ltd, Hebei Provincial Institute of Industrial Technology for Ethnic Medicine Pharmaceutical Preparations, No. 518, Cangsheng Road, High-tech Development Zone, Shijiazhuang City, Hebei Province, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Dec 15;343:126599. doi: 10.1016/j.saa.2025.126599. Epub 2025 Jun 23.
Realize the efficient and sensitive detection of cadmium ions (Cd) and mercury ions (Hg) based on different response modes is of great significance because their large emissions seriously harm the ecological environment and human health. Herein, a novel peptide-based fluorescent probe named DGGC was synthesized and characterized with satisfactory water solubility (100 % aqueous solution) and significantly large Stokes (230 nm). DGGC exhibited high selectivity and excellent sensitivity towards Cd based on fluorescence enhancement response and Hg based on fluorescence quenching response. The limit of detections (LODs) for Cd and Hg were as low as 8.6 nM and 35.1 nM, respectively. The results of fluorescence titration, Job's plot analysis and ESI-HRMS data revealed that DGGC combined with Cd and Hg in a 2:1 stoichiometric ratio. Additionally, the bioimaging results demonstrated that significant discrimination sensitivity of DGGC for imaging of Cd and Hg in living cells and zebrafish larvae. In addition, DGGC showed high precision and accuracy in the quantitative analysis of Cd and Hg in various real samples. Furthermore, DGGC monitored Cd and Hg contamination on the surface of rice, leaves, kiwifruit and pumpkin under 365 nm UV light using naked eyes. The fluorescence change of DGGC after the addition of Cd and Hg was used as an INHIBIT logic gate. More significantly, the smartphone colour recognition App was successfully applied to the semi-quantitative detection of Cd and Hg without the need for a large instrument.
基于不同响应模式实现镉离子（Cd）和汞离子（Hg）的高效灵敏检测具有重要意义，因为它们的大量排放严重危害生态环境和人类健康。在此，合成了一种新型的基于肽的荧光探针DGGC，其具有令人满意的水溶性（100%水溶液）和显著大的斯托克斯位移（230 nm）。DGGC基于荧光增强响应，对Cd表现出高选择性和优异的灵敏度；基于荧光猝灭响应，对Hg也表现出高选择性和优异的灵敏度。Cd和Hg的检测限分别低至8.6 nM和35.1 nM。荧光滴定、Job曲线分析和电喷雾高分辨质谱数据结果表明，DGGC与Cd和Hg以2:1的化学计量比结合。此外，生物成像结果表明，DGGC对活细胞和斑马鱼幼虫中的Cd和Hg成像具有显著的区分灵敏度。此外，DGGC在各种实际样品中对Cd和Hg的定量分析显示出高精度和准确性。此外，DGGC在365 nm紫外光下用肉眼监测了水稻、叶片、猕猴桃和南瓜表面的Cd和Hg污染情况。加入Cd和Hg后DGGC的荧光变化用作一个INHIBIT逻辑门。更重要的是，智能手机颜色识别应用程序成功应用于Cd和Hg的半定量检测，无需大型仪器。
