Torres Anthony, Gaudino Reginald
Cannabis Research Institute, Discovery Partners Institute, University of Illinois System, Chicago, IL, USA.
Front Range Biosciences, Lafayette, CO, USA.
Methods Mol Biol. 2025;2943:73-80. doi: 10.1007/978-1-0716-4642-7_6.
Field-based techniques for genotyping and identification are facilitated by isothermal amplification and detection technology. This chapter focuses on developing a rapid and field-deployable method for early sex identification genotyping in plants. Leveraging the Y-chromosome-specific regions or gene segments, we designed and evaluated an alternative isothermal amplification assay through recombinase polymerase amplification (RPA) to detect male-specific DNA sequences and perform sex determination genotyping.
等温扩增和检测技术推动了基于现场的基因分型和鉴定技术的发展。本章重点在于开发一种快速且可在现场部署的方法，用于植物早期性别鉴定基因分型。利用Y染色体特异性区域或基因片段，我们设计并评估了一种通过重组酶聚合酶扩增（RPA）进行的等温扩增检测方法，以检测雄性特异性DNA序列并进行性别鉴定基因分型。
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