Xu Tengqi, Zhang Zhouchang, Wang Yufan, Zhang Jinyi, Zhang Yiqiong, Han Siqi, Liu Jiaxi, Song Tianjiao, Lin Yanbing
College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China.
College of Life Sciences, Northwest A&F University, Yangling, Shaanxi, 712100, China.
J Environ Manage. 2025 Aug;390:126350. doi: 10.1016/j.jenvman.2025.126350. Epub 2025 Jun 27.
Nitrogen deposition is a crucial component of the global nitrogen cycle and a significant driver of global change. However, current research remains relatively limited regarding the effects of nitrogen deposition on soil properties and microbial communities in grassland ecosystems following long-term natural recovery. Therefore, a simulated nitrogen deposition experiment was conducted in Yunwu Mountain to explore the effects of nitrogen deposition on soil properties and microbial communities. The results showed that nitrogen deposition significantly increased the content of ammonium nitrogen and nitrate nitrogen. Meanwhile, the release of protons caused by the increase in ammonium nitrogen led to a significant decrease in soil pH. Regarding microbial communities, bacterial and fungal communities showed no significant response to nitrogen deposition, whereas archaeal communities were significantly affected. Nitrogen deposition reduced migratory capacity of archaea between the rhizosphere and endosphere, resulting in a significant reduction in the diversity of endophytic archaea. Co-occurrence network analysis demonstrated severe disruption of archaeal network structures under nitrogen deposition. Furthermore, null model analysis revealed that nitrogen deposition altered the community assembly processes of endophytic archaea, driving a gradual shift toward deterministic processes. The results of this study provide key evidence for understanding the ecological hazards of nitrogen deposition and emphasize the need to pay special attention to changes in archaea when studying the long-term effects of nitrogen deposition on ecosystems.
氮沉降是全球氮循环的重要组成部分，也是全球变化的重要驱动因素。然而，目前关于长期自然恢复后氮沉降对草地生态系统土壤性质和微生物群落影响的研究仍然相对有限。因此，在云雾山进行了模拟氮沉降实验，以探究氮沉降对土壤性质和微生物群落的影响。结果表明，氮沉降显著增加了铵态氮和硝态氮的含量。同时，铵态氮增加导致的质子释放使土壤pH值显著降低。关于微生物群落，细菌和真菌群落对氮沉降没有显著响应，而古菌群落受到显著影响。氮沉降降低了古菌在根际和内生菌之间的迁移能力，导致内生古菌多样性显著降低。共现网络分析表明，氮沉降下古菌网络结构受到严重破坏。此外，零模型分析表明，氮沉降改变了内生古菌的群落组装过程，促使其逐渐向确定性过程转变。本研究结果为理解氮沉降的生态危害提供了关键证据，并强调在研究氮沉降对生态系统的长期影响时，需要特别关注古菌的变化。
