Bennypaul H S, St-Jacques S, Schmidt Isabella, Nakata J, Sanderson D S
Center for Plant Health, Canadian Food Inspection Agency, North Saanich, BC V8L 1H3, Canada.
Department of General Sciences, Camosun College, 3100 Foul Bay Rd., Victoria, BC V8P 5J2, Canada.
J Virol Methods. 2025 Dec;338:115211. doi: 10.1016/j.jviromet.2025.115211. Epub 2025 Jun 27.
The yield and quality of apples are affected by diseases caused by bacteria, fungi, viruses, and virus-like organisms. Several viruses are known to cause or be associated with diseases of economic importance which impact apple production. Citrus concave gum-associated virus (CCGaV; order Bunyavirales, family Phenuiviridae, genus Coguvirus) is a negative-stranded RNA virus, with a bipartite genome of negative-stranded RNA1 and an ambisense RNA2. In recent years, CCGaV was identified in apple germplasm collections in a number of countries. Although identified in apple trees showing decline, CCGaV could not be implicated in disease causation due to the presence of other coinfecting viruses. As a precautionary measure, virus elimination programs have included CCGaV in the list of viruses that should be eliminated from the propagative material before distribution to its stakeholders. Detection of tree fruit viruses in woody hosts is difficult as compared to annual crops, due to the low titer, irregular distribution, and the occurrence of mixed infections. Sensitive and specific virus detection methods are critical part of virus elimination programs, first to identify virus infected plants that need to be treated and then to certify virus-free status of after virus elimination treatments. In this study, an RT-qPCR assay was developed for sensitive and specific detection of CCGaV in apples.
苹果的产量和品质受到细菌、真菌、病毒及类病毒生物所引发疾病的影响。已知有几种病毒会引发或与具有经济重要性的疾病相关联，这些疾病会影响苹果生产。柑橘凹皮果胶相关病毒（CCGaV；布尼亚病毒目，芬尼病毒科，柯古病毒属）是一种负链RNA病毒，具有由负链RNA1和双义RNA2组成的二分体基因组。近年来，多个国家的苹果种质资源库中都发现了CCGaV。尽管在表现衰退的苹果树上发现了CCGaV，但由于存在其他共感染病毒，无法确定CCGaV与疾病发生有关。作为预防措施，病毒清除计划已将CCGaV列入在繁殖材料分发给利益相关者之前应清除的病毒名单中。与一年生作物相比，在木本寄主中检测树果病毒较为困难，这是因为病毒滴度低、分布不规则且存在混合感染。灵敏且特异的病毒检测方法是病毒清除计划的关键部分，首先用于识别需要处理的病毒感染植株，然后在病毒清除处理后证明其无病毒状态。在本研究中，开发了一种RT-qPCR检测方法，用于灵敏且特异检测苹果中的CCGaV。
