Arifianto Deny, Astuti Suryani Dyah, Lusiani Baby Mutiara, Zaidan Andi Hamim, Winarno Winarno, Yaqubi Ahmad Khalil, Susilo Yunus, Qadir Karwan Wasman, Syahrom Ardiansyah
Faculty of Science and Technology, Airlangga University, Surabaya, East Java, 60115, Indonesia.
Department of Physics, Faculty of Science and Technology, Airlangga University, Surabaya, 60115, Indonesia.
Lasers Med Sci. 2025 Jun 30;40(1):305. doi: 10.1007/s10103-025-04549-x.
diseases caused by antibiotic-resistant bacteria pose a growing global health challenge. Photodynamic Therapy (PDT), which uses light-activated photosensitizers to kill bacteria, offers a promising alternative. This study evaluates the antibacterial efficacy of gold nanoparticles synthesized from mangosteen peel extract (AuNPs-GML) combined with blue diode laser irradiation against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), two common pathogens.
Four treatments were applied: control (no treatment), AuNPs-GML alone, blue laser irradiation alone (405 nm), and a combination of AuNPs-GML with laser irradiation. Samples were incubated at 37 °C for 24 h before bacterial viability was assessed. Statistical analysis was performed using Two-Way ANOVA and Tukey's post hoc test (p < 0.05).
Results showed that combining AuNPs-GML with blue laser irradiation significantly enhanced bacterial inactivation compared to laser or nanoparticles alone. At 180 seconds irradiation and 2 mM AuNPs-GML concentration, bacterial reduction reached 86.25% for E. coli and 72.84% for S. aureus at an energy density of 3.34 J/cm2.
This study demonstrates the potential of AuNPs-GML activated by blue laser PDT as an effective antibacterial treatment against resistant bacteria.
由抗生素耐药菌引起的疾病对全球健康构成了日益严峻的挑战。光动力疗法（PDT）利用光激活的光敏剂来杀死细菌，提供了一种有前景的替代方法。本研究评估了由山竹果皮提取物合成的金纳米颗粒（AuNPs-GML）与蓝色二极管激光照射联合对两种常见病原体大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）的抗菌效果。
应用了四种处理方式：对照（不处理）、单独使用AuNPs-GML、单独进行蓝色激光照射（405nm）以及AuNPs-GML与激光照射联合。在评估细菌活力之前，将样品在37°C下孵育24小时。使用双向方差分析和Tukey事后检验进行统计分析（p < 0.05）。
结果表明，与单独使用激光或纳米颗粒相比，将AuNPs-GML与蓝色激光照射联合可显著增强细菌灭活效果。在照射180秒且AuNPs-GML浓度为2 mM时，在能量密度为3.34 J/cm2的情况下，大肠杆菌的细菌减少率达到86.25%，金黄色葡萄球菌的细菌减少率达到72.84%。
本研究证明了蓝色激光光动力疗法激活的AuNPs-GML作为一种针对耐药菌的有效抗菌治疗方法的潜力。
