Astuti Suryani Dyah, Rulaningtyas Riries, Putra Alfian P, Arifianto Deny
Department of Physics, Faculty of Science and Technology.
Department of Anatomy, Faculty of Medicine, Airlangga University, Surabaya, Indonesia.
Infect Dis Rep. 2020 Jul 6;12(Suppl 1):8736. doi: 10.4081/idr.2020.8736. eCollection 2020 Jul 7.
Biofilms are able to cause microorganisms to be 80% more resistant to antibiotics. The extracelullar polymeric substance (EPS) in biofilm functions to protect bacteria, making it difficult for antibiotics to penetrate the biofilm layer. This study aims to determine the effectiveness of photodynamic inactivation with blue diode laser to reduce biofilm at various ages of biofilms. The light source is a 403 nm blue diode laser with an energy power of about 27.65±0.01 mW. The study was designed with two groups: Group C was the untreated control group with variations in age of biofilms (0; 6; 11; 17; 24; 32; 40 and 48) hours; Group T was a laser treatment group with variations in age of biofilm and energy density (4.23; 8.46; 12.70; 16.93 and 21.16) J/cm. Biofilm reduction measurement method using ELISA test was performed to calculate OD value. The statistical analysis results of variance showed that there was an influence of biofilm age and irradiation energy density of laser on biofilm reduction. Optical density analysis showed the most optimum biofilm reduction happened when biofilm age is perfectly constructed (about 17 hours) and with 91% reduction. The longer biofilm age lived among those biofilms, the greater the reduction. The results of the Scanning Microscope Electron and fluorescent microscope measurement showed destruction site of the EPS biofilm and bacterial cell death. So, the activated photodynamic with 403 nm laser diode is effective to reduce the biofilm in the maturation phase.
生物膜能够使微生物对抗生素的耐药性提高80%。生物膜中的细胞外聚合物（EPS）起到保护细菌的作用，使抗生素难以穿透生物膜层。本研究旨在确定蓝色二极管激光光动力灭活对不同龄期生物膜减少的有效性。光源为波长403nm的蓝色二极管激光，能量功率约为27.65±0.01mW。本研究设计了两组：C组为未处理的对照组，生物膜龄期有变化（0、6、11、17、24、32、40和48小时）；T组为激光治疗组，生物膜龄期和能量密度有变化（4.23、8.46、12.70、16.93和21.16）J/cm²。采用ELISA试验进行生物膜减少量测量方法以计算OD值。方差统计分析结果表明，生物膜龄期和激光照射能量密度对生物膜减少有影响。光密度分析表明，当生物膜龄期处于完美构建状态（约17小时）时，生物膜减少最为理想，减少率为91%。在这些生物膜中，生物膜龄期越长，减少量越大。扫描电子显微镜和荧光显微镜测量结果显示了EPS生物膜的破坏部位和细菌细胞死亡情况。因此，403nm激光二极管激活的光动力对减少成熟阶段的生物膜有效。
