Mitchell J B, Karawya E, Kinsella T J, Wilson S H
Mutat Res. 1985 Nov;146(3):295-300. doi: 10.1016/0167-8817(85)90071-9.
DNA polymerase beta levels were measured in 4 cell lines of normal human skin fibroblasts and in 5 cell lines of skin fibroblasts from patients with ataxia telangiectasia, an autosomal recessive disease exhibiting marked X-ray sensitivity. The enzyme specific activities for the normal lines were similar and the mean value was 2-fold lower than the mean value for the ataxia lines. With both kinds of cells, the enzyme level did not change as the cultures progressed from logarithmic to stationary phase of growth. Thus, this putative DNA repair enzyme appears to be 'constitutive' in human skin fibroblast lines, and a modest elevation of beta-polymerase activity is associated with ataxia telangiectasia. These results are discussed in the context to current views about DNA-repair enzymes in X-ray-sensitive cultured mammalian cells.
在4株正常人皮肤成纤维细胞系和5株共济失调毛细血管扩张症患者的皮肤成纤维细胞系中测量了DNA聚合酶β的水平。共济失调毛细血管扩张症是一种常染色体隐性疾病，表现出明显的X射线敏感性。正常细胞系的酶比活性相似，其平均值比共济失调细胞系的平均值低2倍。对于这两种细胞，随着培养物从对数生长期进入稳定生长期，酶水平没有变化。因此，这种假定的DNA修复酶在人皮肤成纤维细胞系中似乎是“组成型的”，并且β聚合酶活性的适度升高与共济失调毛细血管扩张症相关。结合当前关于X射线敏感的培养哺乳动物细胞中DNA修复酶的观点对这些结果进行了讨论。
