Zhou Shanlei, Zhang Yu, Casals Eudald, Zeng Muling, Morales-Ruiz Manuel, Liu Qingshi, Zhang Bo, Casals Gregori
Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, Hefei, 230022, People's Republic of China.
Premium Research SL, Guadalajara, 19003, Spain.
Int J Nanomedicine. 2025 Jun 25;20:8191-8207. doi: 10.2147/IJN.S525411. eCollection 2025.
Combining natural antioxidants with nanozymes represents a promising strategy to enhance therapeutic outcomes in oxidative stress-related diseases. This study integrates quercetin (Que), a plant-derived flavonoid with strong antioxidant activity, and cerium oxide nanozymes (CeONZs) into mesoporous silica (mSiO) to enhance therapeutic efficacy and overcome the poor solubility and bioavailability of natural antioxidants.
Large-pore mSiO₂ (11 nm) were synthesized via a sol-gel method to encapsulate cerium oxide nanozymes (CeO₂NZs). Que was loaded using solvent impregnation to obtain (CeO₂/Que)@mSiO₂ nanocomposites. Structural and chemical characterization was performed, and biological evaluations were conducted in A549 cells.
The incorporation of a large mesopore mSiO₂ (11 nm) significantly enhanced Que loading capacity and its sustained release in cell culture media. The (CeO₂/Que)@mSiO₂ nanocomposite demonstrated excellent biocompatibility, effective ROS scavenging, and significant downregulation of inflammatory cytokines (IL-1β, IL-6, TNF-α) compared to free Que.
The (CeO₂/Que)@mSiO₂ nanoplatform offers synergistic antioxidant and anti-inflammatory effects, supporting its potential for treating oxidative stress-related inflammatory conditions.
将天然抗氧化剂与纳米酶相结合是一种有望提高氧化应激相关疾病治疗效果的策略。本研究将槲皮素（Que）（一种具有强抗氧化活性的植物源黄酮类化合物）和氧化铈纳米酶（CeONZs）整合到介孔二氧化硅（mSiO）中，以提高治疗效果，并克服天然抗氧化剂溶解度和生物利用度差的问题。
通过溶胶-凝胶法合成大孔mSiO₂（11纳米）以包封氧化铈纳米酶（CeO₂NZs）。采用溶剂浸渍法负载Que，以获得（CeO₂/Que）@mSiO₂纳米复合材料。进行了结构和化学表征，并在A549细胞中进行了生物学评价。
大孔mSiO₂（11纳米）的加入显著提高了Que的负载能力及其在细胞培养基中的缓释效果。与游离Que相比，（CeO₂/Que）@mSiO₂纳米复合材料表现出优异的生物相容性、有效的活性氧清除能力以及炎症细胞因子（IL-1β、IL-6、TNF-α）的显著下调。
（CeO₂/Que）@mSiO₂纳米平台具有协同抗氧化和抗炎作用，支持其治疗氧化应激相关炎症性疾病的潜力。
