Galiniak Sabina, Biesiadecki Marek, Podgórski Rafał, Dąbek Natalia, Gramatyka-Drążek Ewa, Gaweł Ewelina, Ranke Michael B, Flehmig Bertram, Wabitsch Martin, Brandt Stephanie, Petriczko Elżbieta, Wójcik Małgorzata, Małecka-Tendera Ewa, Bik-Multanowski Mirosław, Zachurzok Agnieszka, Mazur Artur
Endocr Connect. 2025 Jul 16;14(7). doi: 10.1530/EC-25-0194. Print 2025 Jul 1.
Childhood severe obesity (SO) is a growing public health concern. Leptin (LEP) and its biologically active fraction (BioLEP) play a key role in energy balance and body mass regulation. Understanding their relationship with anthropometric and metabolic parameters is crucial for improving SO treatment. This study analyzed total LEP, BioLEP, and the BioLEP/LEP ratio in children with SO and their associations with body composition, lipid profile, and pubertal development. The study included 461 children (245 girls and 216 boys) aged 0-19 years with SO. Anthropometric parameters, metabolic indices, and pubertal stages (Tanner scale) were assessed. LEP and BioLEP concentrations were measured using ELISA, and the BioLEP/LEP ratio was calculated. The median LEP concentration was 56.53 ng/mL, and BioLEP was 53.66 ng/mL, with a BioLEP/LEP ratio of 0.94. Girls had significantly higher LEP (64.40 vs 49.66 ng/mL, P < 0.001) and BioLEP (59.90 vs 46.93 ng/mL, P < 0.001) than boys. LEP and BioLEP correlated positively with BMI, waist and hip circumference, and fat mass percentage, while negatively with waist-to-hip ratio and fat-free mass. The BioLEP/LEP ratio correlated positively with insulin and lipid profile parameters (total cholesterol, HDL, and triglycerides). LEP and BioLEP play a significant role in SO, particularly in fat mass regulation. The BioLEP/LEP ratio is more closely linked to lipid metabolism. These findings provide insights into the hormonal and metabolic profile of SO, supporting targeted diagnostic and therapeutic approaches.
儿童重度肥胖是一个日益严重的公共卫生问题。瘦素（LEP）及其生物活性部分（BioLEP）在能量平衡和体重调节中起关键作用。了解它们与人体测量和代谢参数的关系对于改善重度肥胖的治疗至关重要。本研究分析了重度肥胖儿童的总LEP、BioLEP以及BioLEP/LEP比值，并探讨了它们与身体成分、血脂谱和青春期发育的关联。该研究纳入了461名0至19岁的重度肥胖儿童（245名女孩和216名男孩）。评估了人体测量参数、代谢指标和青春期阶段（坦纳分期）。采用酶联免疫吸附测定法（ELISA）测量LEP和BioLEP浓度，并计算BioLEP/LEP比值。LEP浓度中位数为56.53 ng/mL，BioLEP为53.66 ng/mL，BioLEP/LEP比值为0.94。女孩的LEP（64.40 vs 49.66 ng/mL，P < 0.001）和BioLEP（59.90 vs 46.93 ng/mL，P < 0.001）显著高于男孩。LEP和BioLEP与体重指数、腰围和臀围以及脂肪质量百分比呈正相关，而与腰臀比和去脂体重呈负相关。BioLEP/LEP比值与胰岛素和血脂谱参数（总胆固醇、高密度脂蛋白和甘油三酯）呈正相关。LEP和BioLEP在重度肥胖中起重要作用，尤其是在脂肪质量调节方面。BioLEP/LEP比值与脂质代谢的关系更为密切。这些发现为重度肥胖的激素和代谢特征提供了见解，支持针对性的诊断和治疗方法。
