Kolta M G, Shreve P, De Souza V, Uretsky N J
Neuropharmacology. 1985 Sep;24(9):823-9. doi: 10.1016/0028-3908(85)90032-2.
These experiments were designed to examine the time course of development of the enhanced stereotyped behavioral response to amphetamine after withdrawal from chronic pretreatment with amphetamine and to determine whether this time course correlates with that of the enhancement in the amphetamine-induced stimulation of the release of dopamine (DA) from striatal slices. Rats were pretreated with amphetamine (5 mg/kg, i.p.) or saline, twice daily for 5 consecutive days. At 3, 15 and 30 days after withdrawal of the drug the stereotyped behavioral response and the release of endogenous DA from slices of striatum in response to a challenge dose of amphetamine were measured. At all 3 times tested, the stereotyped behavioral response to the challenge dose of amphetamine was enhanced in the rats pretreated with amphetamine, with the greatest degree of enhancement seen at 15 and 30 days after withdrawal of the drug. At these times, the responses were associated with a significant attenuation in the stimulation of locomotor activity produced by the challenge dose of amphetamine, which was probably related to the enhanced stereotyped behavioral response. Amphetamine stimulated the release of endogenous DA from slices of striatum in rats pretreated with saline and amphetamine. However, the release of endogenous DA from slices of rats pretreated with amphetamine was significantly greater than that of saline-pretreated rats at 15 and 30 days after withdrawal of the drug, but not at 3 days after withdrawal. Thus, pretreatment with amphetamine resulted in enhanced behavioral and biochemical responses to amphetamine which increased over time after withdrawal of the drug.(ABSTRACT TRUNCATED AT 250 WORDS)
这些实验旨在研究大鼠在长期使用苯丙胺预处理后戒断，对苯丙胺增强的刻板行为反应的发展时间进程，并确定该时间进程是否与苯丙胺诱导的纹状体切片中多巴胺（DA）释放增强的时间进程相关。大鼠连续5天每天两次接受苯丙胺（5mg/kg，腹腔注射）或生理盐水预处理。在停药后3天、15天和30天，测量对苯丙胺激发剂量的刻板行为反应以及纹状体切片中内源性DA的释放。在所有3个测试时间点，接受苯丙胺预处理的大鼠对苯丙胺激发剂量的刻板行为反应均增强，在停药后15天和30天增强程度最大。在这些时间点，这些反应与苯丙胺激发剂量引起的运动活性刺激的显著减弱相关，这可能与增强的刻板行为反应有关。苯丙胺刺激了接受生理盐水和苯丙胺预处理大鼠的纹状体切片中内源性DA的释放。然而，在停药后15天和30天，接受苯丙胺预处理大鼠的纹状体切片中内源性DA的释放显著高于接受生理盐水预处理的大鼠，但在停药后3天并非如此。因此，苯丙胺预处理导致对苯丙胺的行为和生化反应增强，且在停药后随时间增加。（摘要截短至250字）
