Shohel Mustakin Ahmed, Kabir Md Humayan, Shaker Ivvala Anand, Siddika Sumaiya, Rahman Sheikh Arafat, Siddiquea Sumaiya, Mahat Nirmal Chandra, Islam Tarique Muhammad Tawabul, Tang Md Abul Kashem
Department of Food & Nutrition, Faculty of Applied Science, Parul University, Waghodia, India.
Department of Applied Nutrition and Food Technology, Islamic University, Kushtia, Bangladesh.
J Pharmacopuncture. 2025 Jun 30;28(2):127-137. doi: 10.3831/KPI.2025.28.2.127.
Diarrheal diseases remain a significant global health concern, with rising resistance to traditional pharmaceutical treatments. , a tropical fruit, is known for its medicinal properties, but its seed, which is often discarded as waste, has not been extensively studied for its potential health benefits. This study investigates the antidiarrheal effects of seed extract (LCSE).
Ethanolic extract was prepared from dried, powdered seeds. Acute oral toxicity was conducted by administering a single 2,000 mg/kg dose to female Swiss albino mice. Mice were divided into five groups (control, positive control with Loperamide, and test groups receiving 100, 200, and 400 mg/kg of LCSE) for the antidiarrheal assay. Diarrhea was induced with castor oil, and parameters such as onset of diarrhea, fecal output, gastrointestinal motility, and enteropooling were measured. The statistical analysis was conducted in GraphPad Prism version 10.1.2 using one-way ANOVA followed by a Dunnett multiple comparison test.
seed extract (LCSE) significantly (p < 0.001) delayed the onset of diarrhea, reduced the frequency of defecation, and decreased fecal weight in the castor oil-induced diarrhea model at all doses (100, 200, and 400 mg/kg). Additionally, LCSE significantly (p < 0.001) reduced total and wet fecal output, inhibited gastrointestinal motility (p < 0.001) in the charcoal meal test, and notably (p < 0.001) decreased the volume and weight of intestinal contents in the enteropooling assay. The antidiarrheal index (ADI) showed a dose-dependent increase, with the highest ADI recorded at 400 mg/kg (189.42%), slightly surpassing the standard drug, loperamide (160.14%).
The finding supports the antidiarrheal potential of LCSE holds potent antidiarrheal properties, positioning it as a promising, sustainable alternative to modern medications.
腹泻病仍然是一个重大的全球健康问题，对传统药物治疗的耐药性不断上升。[水果名称]是一种热带水果，以其药用特性而闻名，但其种子常作为废物丢弃，尚未对其潜在的健康益处进行广泛研究。本研究调查了[水果名称]种子提取物（LCSE）的止泻作用。
从干燥、磨粉的[水果名称]种子中制备乙醇提取物。通过对雌性瑞士白化小鼠单次给予2000mg/kg剂量进行急性口服毒性试验。将小鼠分为五组（对照组、用洛哌丁胺作为阳性对照，以及接受100、200和400mg/kg LCSE的试验组）进行止泻试验。用蓖麻油诱导腹泻，并测量腹泻发作、粪便输出、胃肠动力和肠内积液等参数。使用GraphPad Prism 10.1.2软件进行统计分析，采用单因素方差分析，随后进行Dunnett多重比较检验。
在蓖麻油诱导的腹泻模型中，[水果名称]种子提取物（LCSE）在所有剂量（100、200和400mg/kg）下均显著（p<0.001）延迟腹泻发作，降低排便频率，并减轻粪便重量。此外，LCSE在炭末试验中显著（p<0.001）减少总粪便输出量和湿粪便输出量，抑制胃肠动力（p<0.001），并且在肠内积液试验中显著（p<0.001）降低肠内容物的体积和重量。[水果名称]止泻指数（ADI）呈剂量依赖性增加，在400mg/kg时记录到最高ADI（189.42%），略超过标准药物洛哌丁胺（160.14%）。
该研究结果支持LCSE具有止泻潜力，具有强大的止泻特性，使其成为现代药物有前景的可持续替代品。
