Waongo Blaise, Ndayishimiye Libère, Tapsoba François, Zongo Wend-Soo Abel, Li Jinquan, Savadogo Aly
Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou, Burkina Faso.
National Key Laboratory of Agricultural Microbiology, Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, College of Biomedicine and Health, Huazhong Agricultural University, Wuhan, China.
Front Microbiol. 2025 Jun 16;16:1515483. doi: 10.3389/fmicb.2025.1515483. eCollection 2025.
Experimental studies, though often very costly, lead to the discovery of known antimicrobial products. Yet, pathogenic microorganisms are proving increasingly resistant to pre-existing antimicrobial molecules, and this is a cause for worldwide concern. Therefore, it is necessary to search for new molecules that could serve as alternatives in the food, medical and agricultural sectors. Thus, 123 complete genomes of strains isolated from soil and fermented foods were analyzed and annotated using bioinformatics prediction and characterization tools. The view was to discover new gene clusters for the biosynthesis of non-ribosomal peptides (lipopeptides, siderophores, antibiotics). This study revealed that 83% of the genomes analyzed possess biosynthetic gene clusters for the production of siderophore bacillibactin, 61% for surfactins, 37% for fengycins, 23% for iturins, 15% for kurstakins and 3% for bacitracin. Besides, seven new biosynthetic gene clusters coding Non Ribosomal Peptide Synthetases (NRPS) have been identified in ATR2, DSYZ, CGMCC11640, HM618, WF02, CMCC P0011, CMCC P0021, SJ-10 and CMF9. The results of this study revealed a significant potential of the genus to produce new non-ribosomally synthesized peptides. Now, these predicted new antimicrobial molecules can be easily studied experimentally as many new gene clusters have been identified.
实验研究虽然往往成本很高，但能促成已知抗菌产品的发现。然而，致病微生物对现有的抗菌分子的耐药性越来越强，这已成为全球关注的问题。因此，有必要寻找可在食品、医疗和农业领域用作替代品的新分子。于是，利用生物信息学预测和表征工具，对从土壤和发酵食品中分离出的菌株的123个完整基因组进行了分析和注释。目的是发现用于非核糖体肽（脂肽、铁载体、抗生素）生物合成的新基因簇。这项研究表明，83%的分析基因组拥有用于生产铁载体杆菌肽的生物合成基因簇，61%用于表面活性素，37%用于丰原素，23%用于伊枯草菌素，15%用于库斯塔金，3%用于杆菌肽。此外，在ATR2、DSYZ、CGMCC11640、HM618、WF02、CMCC P0011、CMCC P0021、SJ - 10和CMF9中已鉴定出七个编码非核糖体肽合成酶（NRPS）的新生物合成基因簇。这项研究的结果揭示了该属产生新的非核糖体合成肽的巨大潜力。现在，由于已鉴定出许多新的基因簇，这些预测的新抗菌分子可以很容易地进行实验研究。
