Yan Hanwen, Han Guohao, Gu Tiantian, Shi Zhipeng, Cao Lijun, Wang Jing, Liu Hong, Wang Chenlong, Zhuo Shiyu, Li Lihui, An Diaoguo
Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Theor Appl Genet. 2025 Jul 1;138(7):167. doi: 10.1007/s00122-025-04955-3.
A wheat-rye 2R (2D) substitution line with PmYT9 conferring powdery mildew resistance was characterized. PmYT9 was mapped to a 14. 55 Mb interval on 2RL. A homozygous translocation line carrying PmYT9 was developed. Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), poses a significant threat to wheat (Triticum aestivum L.) production. The identification and utilization of novel resistance genes from wheat relatives are an effective strategy for sustainable disease management. Rye (Secale cereale L. RR), a tertiary gene pool of wheat, harbors abundant genetic diversity. In this study, we developed a novel wheat-rye derivative line, YT9, by crossing hexaploid triticale 09R1-16 with wheat breeding line PB9. Genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), and agronomic trait evaluations confirmed that YT9 was a stable 2R (2D) substitution and T1BL·1RS translocation line with favorable performance. Resistance phenotyping and microscopic observation of fungal growth revealed that YT9 exhibited resistance to Bgt from the three-leaf stage. Genetic analysis localized the resistance gene to chromosome arm 2RL of rye, designated PmYT9. To map PmYT9, Coγ-ray irradiation was employed to induce chromosomal structure variants. Integrated GISH, molecular marker analysis, and disease response assessment delimited PmYT9 to a 14.55 Mb interval (831.45-846.00 Mb) on the Lo7 rye reference genome, flanked by markers SW11163 and X2RL78. Additionally, a homozygous T7DS·7DL-2RL translocation line carrying PmYT9 was developed. This study expands the genetic diversity of wheat powdery mildew resistance, provides elite germplasm for wheat resistance breeding, and establishes a foundation for the molecular cloning of PmYT9.
对携带抗白粉病基因PmYT9的小麦-黑麦2R(2D)代换系进行了鉴定。PmYT9被定位到2RL上一个14.55 Mb的区间。培育出了携带PmYT9的纯合易位系。由小麦白粉病菌（Bgt）引起的白粉病对小麦（普通小麦）生产构成重大威胁。从小麦近缘种中鉴定和利用新的抗性基因是实现可持续病害治理的有效策略。黑麦（节节麦RR）作为小麦的三级基因库，拥有丰富的遗传多样性。在本研究中，我们通过将六倍体小黑麦09R1-16与小麦育种系PB9杂交，培育出了一个新的小麦-黑麦衍生系YT9。基因组原位杂交（GISH）、荧光原位杂交（FISH）和农艺性状评价证实，YT9是一个表现良好的稳定2R(2D)代换和T1BL·1RS易位系。抗性表型分析和真菌生长的显微镜观察表明，YT9从三叶期起就对白粉病菌表现出抗性。遗传分析将抗性基因定位到黑麦的2RL染色体臂上，命名为PmYT9。为了定位PmYT9，采用Coγ射线辐照诱导染色体结构变异。综合GISH、分子标记分析和病害反应评估，将PmYT9定位到Lo7黑麦参考基因组上一个14.55 Mb（831.45-846.00 Mb）的区间，两侧标记为SW11163和X2RL78。此外，还培育出了携带PmYT9的纯合T7DS·7DL-2RL易位系。本研究拓宽了小麦抗白粉病的遗传多样性，为小麦抗性育种提供了优良种质，并为PmYT9的分子克隆奠定了基础。
