Development and cytological characterization of wheat- translocation lines with novel stripe rust resistance gene.

Wheat stripe rust is a destructive disease in many cool and temperate regions around the world. Exploiting novel sources of resistance can provide wheat cultivars with robust and durable resistance to stripe rust. The wheat- addition line TAI-14 was proven to carry a stripe rust resistance gene (named as ) on the alien chromosome. In order to transfer the resistance gene to wheat, wheat- translocation lines were created by irradiating the pollen of the line TAI-14. We totally obtained 153 wheat- translocation lines, among which the long alien segmental translocation line Zhongke 78 and the intercalary translocation line Zhongke 15 not only showed good integrated agronomic traits but also were identified as highly resistant to stripe rust in both seedling and adult plant stages. The alien chromatin in Zhongke 15 was identified as an insertion into the satellite of chromosome 6B, a type of translocation never reported before in chromosome engineering. By screening Simple Sequence Repeat (SSR) and Expressed Sequence Tag (EST) markers as well as the markers developed from RNA-sequencing (RNA-Seq) data, 14 markers were identified specific for the alien chromosome and a physical map was constructed. Both Zhongke 78 and Zhongke 15 could be used as a novel source of stripe rust resistance for wheat breeding, and the linked marker T14K50 can be used for molecular marker-assisted breeding. Finally, based on the karyotype, reaction to stripe rust, and genome resequencing data of different wheat- translocation lines, the stripe rust resistance gene was located to an 88.1 Mb interval from 636.7 to 724.8 Mb on chromosome 19 corresponding to 7J or 7J.